Probe:	POEM cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Labeled probes were alkaline-hydrolyzed and then purified.

Structure	Level	Pattern	Image	Note
TS24: visceral epithelium of maturing renal corpuscle	Strong		4B
TS24: urothelium of ureter	Strong	Regionally restricted	4B	Expression is in most of the epithelium of the ureter branches.
TS24: collecting duct	Present	Regionally restricted	4B	Expression is in the epithelium.
TS24: comma-shaped body	Weak	Regionally restricted	4B	Expression is toward the peripheral region of the kidney.
TS24: s-shaped body	Weak	Regionally restricted	4B	Expression is toward the peripheral region of the kidney.

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Present	Regionally restricted	4D	Expression is in the developing follicular epithelial cells and potential parafollicular cells.
TS24: parathyroid gland	Present	Regionally restricted	4D	Expression is in the developing parenchymal cells of cell cords and is absent in the capsule.
TS24: blood vessel	Absent		4D

Structure	Level	Pattern	Image	Note
TS24: stellate reticulum	Present		4G
TS24: outer enamel epithelium	Present	Regionally restricted	4G	Expression is in the outer part on the frontal side of the outer enamel epithelium and its stalk region.
TS24: inner enamel epithelium	Present	Regionally restricted	4G
TS24: molar dental papilla	Absent		4G

Structure	Level	Pattern	Image	Note
TS24: mandible ramus	Present	Regionally restricted	4J	Expression is high in condensed mesenchymal cells and weak in osteoblasts lining the trabecular bone.

Structure	Level	Pattern	Image	Note
TS24: vertebra	Weak	Regionally restricted	5B	Expression is in osteoblasts and in the cells surrounding bony collar.

Structure	Level	Pattern	Image	Note
TS24: tubotympanic recess	Present		5E
TS24: pharyngo-tympanic tube epithelium	Present	Regionally restricted	5E	Expression is on the outer side.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present	Regionally restricted	5H	Expression is several support cells and sensory epithelium (developing hair cells).

Structure	Level	Pattern	Image	Note
TS24: diaphragm dome	Present		5K
TS24: tendon	Absent		5K

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		5N	Expression is higher around the nucleus, especially in the region just under the lingual aponeurosis and near the lingual septum, where the muscle makes contact.
TS24: tongue epithelium	Present	Regionally restricted	5N	Expression is in the mucosal epithelium.
TS24: tongue	Present	Regionally restricted	5N	Expression is absent in the lingual aponeurosis and lingual aorta.

Structure	Level	Pattern	Image	Note
TS24: stomach wall	Present	Regionally restricted	5Q	Expression is in the inner muscle layer.
TS24: gut	Trace	Regionally restricted	5Q	Expression was barely detected from the duodenum to the rectum.

Structure	Level	Pattern	Image	Note
TS24: pineal gland	Present		6B
TS24: 3rd ventricle choroid plexus	Present	Regionally restricted	6B	Expression is in the dorsal region of the choroid plexus within the pineal recess of the third ventricle.
TS24: 4th ventricle choroid plexus	Weak		6B
TS24: lateral ventricle choroid plexus	Weak		6B

Structure	Level	Pattern	Image	Note
TS24: neurohypophysis	Present	Regionally restricted	6E	Expression is in the nerve fibers and in the cell bodies.
TS24: 3rd ventricle choroid plexus	Present		6E
TS24: adenohypophysis	Present	Regionally restricted	6E	Expression is higher in the cells near the lumen.
TS24: adenohypophysis pars intermedia	Present	Regionally restricted	6E	Expression is higher in the cells near the lumen.

Structure	Level	Pattern	Image	Note
TS24: midbrain tegmentum	Present	Regionally restricted	6H	Expression is in the cells and in their fibers in the medial and lateral regions of the periaquaductal gray area.
TS24: dorsal thalamus	Present		6H
TS24: pretectum	Present		6H
TS24: hypothalamus	Present	Regionally restricted	6H	Expression is in the medial, and in the caudal portion of the lateral hypothalamus.
TS24: superior colliculus	Present	Regionally restricted	6H	Expression is in the cell bodies and their fibers.
TS24: pons	Present	Regionally restricted	6H	In the dorsal portion of pons, expression is in cell bodies and their fibers and is distributed from the rostral region to the caudal region.
TS24: medulla oblongata	Present	Scattered	6H

Structure	Level	Pattern	Image	Note
TS24: back skin	Present	Regionally restricted	6K	Expression is in in the basal layer and muscle layer.
TS24: epidermis	Present	Regionally restricted	6K	Expression is in in the basal layer.

Structure	Level	Pattern	Image	Note
TS24: female external genitalia	Very strong	Regionally restricted	6N	Expression is in the cavernous body and in the basal layer of the glans clitoridis.

Structure	Level	Pattern	Image	Note
TS24: nasopharynx epithelium	Present		6Q
TS24: trachea epithelium	Present		6Q
TS24: respiratory system smooth muscle	Present	Regionally restricted	6Q	Expression is in the smooth muscle of the trachea.
TS24: trachea	Present	Regionally restricted	6Q	Expression is in the smooth muscle of the trachea.

Structure	Level	Pattern	Image	Note
TS24: chondrocranium	Present	Regionally restricted		Expression is high in condensed mesenchymal cells and weak in osteoblasts lining the trabecular bone.
TS24: esophagus muscularis	Present			Expression is in both the inner and outer muscle layers.
TS24: alveolar system	Present	Regionally restricted		Expression is in cells surrounding the developing alveoli.
TS24: spleen	Weak	Single cells
TS24: adrenal gland	Absent
TS24: arterial system smooth muscle	Absent
TS24: blood vessel endothelium	Absent
TS24: cartilage	Absent
TS24: ganglion	Absent
TS24: heart atrium	Absent
TS24: heart ventricle	Absent
TS24: liver	Absent
TS24: ovary	Absent
TS24: pancreas	Absent
TS24: spinal cord	Absent
TS24: thymus	Absent
TS24: vascular system	Absent
TS24: venous system smooth muscle	Absent

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present
TS28: spleen white pulp	Present
TS28: liver	Absent

Structure	Level	Pattern	Image	Note
TS28: spleen	Absent