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Gene Expression Data
Assay Details
Assay
Reference: J:285737 Cui W, et al., MCRS1 is essential for epiblast development during early mouse embryogenesis. Reproduction. 2020 Jan;159(1):1-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6404960
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CDX2-88)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen +/+: embryonic day 3.5 (more )
Note: Blastocysts were flushed out at E3.5, then fixed directly for staining. Triple labeled: white - Cdx2; red - Nanog (assay MGI:6404983); green - Sox17 (assay MGI:6404981).
Structure Level Pattern Image Note
TS4: trophectoderm Present S4_Merge Mcrs1+/+ , S4_Cdx2 Mcrs1+/+
Specimen +/-: embryonic day 3.5; Mcrs1tm1.1(KOMP)Vlcg/Mcrs1+ (more )
Note: Blastocysts were flushed out at E3.5, then fixed directly for staining. Triple labeled: white - Cdx2; red - Nanog (assay MGI:6404983); green - Sox17 (assay MGI:6404981).
Structure Level Pattern Image Note
TS4: trophectoderm Present S4_Merge Mcrs1+/- , S4_Cdx2 Mcrs1+/- Similar as in the wild-type.
Specimen -/-: embryonic day 3.5; Mcrs1tm1.1(KOMP)Vlcg/Mcrs1tm1.1(KOMP)Vlcg (more )
Note: Blastocysts were flushed out at E3.5, then fixed directly for staining. Triple labeled: white - Cdx2; red - Nanog (assay MGI:6404983); green - Sox17 (assay MGI:6404981).
Structure Level Pattern Image Note
TS4: trophectoderm Present S4_Cdx2 Mcrs1-/- , S4_Merge Mcrs1-/- Similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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