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Gene Expression Data
Assay Details
Assay
Reference: J:284089 Mattiske D, et al., A novel long non-coding RNA, Leat1, causes reduced anogenital distance and fertility in female mice. Differentiation. 2019 Dec 4;112:1-6
Assay type: RT-PCR
MGI Accession ID: MGI:6404122
Gene symbol: Efnb2
Gene name: ephrin B2
Probe: Efnb2-pE, Efnb2-pF
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Actin and Hprt were used as housekeeping genes for normalization.
Results Image: 1B
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
E12.5 E12.5 TS20: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E13.5 E13.5 TS21: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E14.5 E14.5 TS22: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E15.5 E15.5 TS23: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E16.5 E16.5 TS24: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E17.5 E17.5 TS25: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E18.5 E18.5 TS26: genital tubercle Ambiguous 0.8 µg; total RNA FVB/NJ Female
Notes:
(a) Expression levels in wild type peaked at E14.5 with a smaller peak at E17.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory