Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149502
Hill P, et al., A SHH-independent regulation of Gli3 is a significant determinant of anteroposterior patterning of the limb bud. Dev Biol. 2009 Apr 15;328(2):506-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6404107
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 probe21
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
7A:
embryonic day 11.5
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7A
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Anteroposterior asymmetry of expression. Lowest expression in posterior part of limb bud.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Strong |
Widespread |
7B
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High level of expression throughout limb bud.
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Specimen
7C:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Weak |
Regionally restricted |
7C
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Authors report weakened but clear anteroposterior asymmetry.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
7D
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Authors report expression was unaffected by combined loss of both Gli3 and Shh.
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Specimen
7E:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Weak |
Regionally restricted |
7E
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Authors report weakened but clear anteroposterior asymmetry.
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7F
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Expression tended to be slightly posteriorly expanded; Solid anterior prevalence of expression persisted.
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H * C57BL/6
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Mutant Allele(s):
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Gli3tm1Urt/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7G
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Expression indistinguishable from wild type.
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Specimen
7H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C3H * C57BL/6
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Mutant Allele(s):
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Gli3tm1Urt/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7H
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Expression indistinguishable from wild type.
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Analysis Tools