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Gene Expression Data
Assay Details
Assay
Reference: J:149502 Hill P, et al., A SHH-independent regulation of Gli3 is a significant determinant of anteroposterior patterning of the limb bud. Dev Biol. 2009 Apr 15;328(2):506-16
Assay type: RNA in situ
MGI Accession ID: MGI:6404105
Gene symbol: Tbx2
Gene name: T-box 2
Probe: Tbx2 probe15
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5J: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: handplate mesenchyme Present Regionally restricted 5J Authors report posterior autopodial expression.
Specimen 5K: embryonic day 11.5; Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS19: handplate mesenchyme Present Regionally restricted 5K Intensity of expression in distal mesenchyme weaker than in wild type.
Specimen 5L: embryonic day 11.5; Gli3tm1Urt/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS19: handplate mesenchyme Present Regionally restricted 5L Intensity of expression in distal mesenchyme weaker than in wild type.
Specimen 5M: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hand mesenchyme Present Regionally restricted 5M Proximal anterior and proximal posterior domains of expression.
TS20: interdigital region between forelimb digits 4 and 5 mesenchyme Strong 5M
TS20: forelimb digit 5 mesenchyme Present Regionally restricted 5M Expression in mesenchyme at tip of digit 5.
TS20: forelimb digit 4 mesenchyme Trace Regionally restricted 5M Expression in mesenchyme at tip of digit 5.
Specimen 5N: embryonic day 12.5; Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS20: interdigital region between forelimb digits 4 and 5 mesenchyme Strong 5N
TS20: hand mesenchyme Present Regionally restricted 5N Expression in posterior-most interdigital region unaffected.
TS20: forelimb digit mesenchyme Present Regionally restricted 5N Expression in distal mesenchyme extended fully alon anterior posterior axis of limb bud.
Specimen 5O: embryonic day 12.5; Gli3tm1Urt/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS20: hand mesenchyme Present Regionally restricted 5O Expression essentially as in wild type.
TS20: interdigital region between forelimb digits 4 and 5 mesenchyme Strong 5O
TS20: forelimb digit mesenchyme Present Regionally restricted 5O Slight expansion of distal expression domain which in some cases extended up to central region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory