Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149502
Hill P, et al., A SHH-independent regulation of Gli3 is a significant determinant of anteroposterior patterning of the limb bud. Dev Biol. 2009 Apr 15;328(2):506-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6404102
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Gene symbol:
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Hand2
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Gene name:
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heart and neural crest derivatives expressed 2
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Probe:
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dHand probe22
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4J:
embryonic day 11.5
(more  )
Specimen
4J:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4K:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4L:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H * C57BL/6
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Mutant Allele(s):
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Gli3tm1Urt/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
4L
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Expression generally normal. Distal expression slightly reduced in some cases, but usually normal.
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Analysis Tools
