Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149502
Hill P, et al., A SHH-independent regulation of Gli3 is a significant determinant of anteroposterior patterning of the limb bud. Dev Biol. 2009 Apr 15;328(2):506-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6404099
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Gene symbol:
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Pax9
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Gene name:
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paired box 9
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Probe:
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Pax9 probe19
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4D:
embryonic day 12.5
(more  )
Specimen
4D:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
4D
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Very prominent anterior domain of expression. Much weaker posterior domain.
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Specimen
4E:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
4E
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Persistent posterior expression. Anterior expression drastically reduced.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H * C57BL/6
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Mutant Allele(s):
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Gli3tm1Urt/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
4F
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Authors report normal intensity of expression with strong anterior and weak posterior expression domains.
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Analysis Tools
