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Gene Expression Data
Assay Details
Assay
Reference: J:284140 Frum T, et al., TEAD4, YAP1 and WWTR1 prevent the premature onset of pluripotency prior to the 16-cell stage. Development. 2019 Sep 6;146(17):dev179861
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6403730
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog (RCAB0002P-F/ RCAB001P)
Detection system: Secondary antibody
Results
Specimen 1F non-mutant: embryonic day 3.75 (more )
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS5: inner cell mass Present Ubiquitous 1F Merge upper , 1F Nanog upper
Specimen 1F Oct4 null: embryonic day 3.75; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS5: inner cell mass Present Ubiquitous 1F Nanog lower , 1F Merge lower
Specimen 1H non-mutant: embryonic day 4.25 (more )
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS6: epiblast Present 1H Merge upper , 1H Nanog upper
Specimen 1H Oct4 null: embryonic day 4.25; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS6: epiblast Present 1H Merge lower , 1H Nanog lower The number of expressing cells is reduced in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory