Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60156
Lee K, et al., Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology. 1996 Nov;137(11):5109-18
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6403330
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Gene symbol:
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Bcl2
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Gene name:
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B cell leukemia/lymphoma 2
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Probe:
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bcl-2 cDNA
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Tissue fixed in 10% formalin.
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Results
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Specimen
8a/b:
embryonic day 16.5
(more  )
Note: proximal end of tibia
Specimen
8a/b:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Age Note:
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Although the text of the paper refers to this specimen as E17.5, the legend refers to it as E16.5, which is more consistent with the rest of the paper.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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proximal end of tibia
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
8a/b
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Strong expression in the lower proliferating zone of epiphyseal cartilage; nearly undetectable levels of expression in hypertrophic chondrocytes.
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Specimen
8c/d:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pthlhtm1Hmk/Pthlhtm1Hmk
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Age:
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embryonic day 16.5
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Age Note:
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Although the text of the paper refers to this specimen as E17.5, the legend refers to it as E16.5, which is more consistent with the rest of the paper.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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proximal end of tibia
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
8c/d
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In the mutant the area of expression is shorter and less well-demarcated.
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Analysis Tools
