Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:60156
Lee K, et al., Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology. 1996 Nov;137(11):5109-18
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6403306
|
|
Gene symbol:
|
Pthlh
|
|
Gene name:
|
parathyroid hormone-like peptide
|
|
Antibody:
|
anti-PTHrP
|
|
Detection system:
|
Secondary antibody coupled to horseradish peroxidase
|
|
Assay notes:
|
Tissue fixed in 10% formalin.
|
|
|
Results
|
Specimen
3Aa:
(close  )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Pthlhtm1Hmk/Pthlhtm1Hmk
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
proximal end of tibia
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Absent |
|
3Aa
|
|
Specimen
3Ab:
embryonic day 16.5
(more  )
Note: proximal end of tibia
Specimen
3Ab:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
proximal end of tibia
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
3Ab
|
Stongest expression is present in a thin layer of chondrocytes immediately above the hypertrophic zone; it is less abundant in the hypertrophic zone. In contrast to the mRNA, the protein is not detectable in either the proliferating chondroyctes or in the perichondrium.
|
|
Analysis Tools
