Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:60156
Lee K, et al., Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology. 1996 Nov;137(11):5109-18
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6403293
|
|
Gene symbol:
|
Pthlh
|
|
Gene name:
|
parathyroid hormone-like peptide
|
|
Probe:
|
PTHrP cDNA
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
Assay notes:
|
Tissue fixed in 10% formalin.
|
|
|
Results
|
Specimen
2a/b:
embryonic day 17.5
(more  )
Specimen
2a/b:
(close  )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metatarsal bone |
Present |
Regionally restricted |
2a/b
|
Expression is highest in the perichondrium adjacent to the uncavitated joint.
|
Specimen
2c:
embryonic day 17.5
(more )
Note: distal end of tibia
Specimen
2c:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
distal end of tibia
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Present |
Regionally restricted |
2f
,
2g
,
2c
|
Expression is seen in the outer layer of the perichondrium and chondocytes; relatively few of those chondrocytes are proliferating.
|
Specimen
2d:
embryonic day 17.5
(more )
Note: distal end of tibia
Specimen
2d:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
distal end of tibia
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Present |
Regionally restricted |
2d
|
Expression is seen in the outer layer of the perichondrium and chondocytes; relatively few of those chondrocytes are proliferating.
|
Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: bone |
Present |
Regionally restricted |
|
In endochondral bones expression is seen in the perichondrium adjacent to the uncavitated joint; in chondrocytes, particuarly those near the epiphyseal ends of long bones; and in sites of tendinous insertions.
|
Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: endochondral bone |
Present |
Regionally restricted |
|
In endochondral bones expression is seen the perichondrium adjacent to the uncavitated joint; in chondrocytes, particuarly those near the epiphyseal ends of long bones; and in sites of tendinous insertions.
|
|
Analysis Tools