Gene Expression Data
Assay Details
Assay
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Reference:
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J:60156
Lee K, et al., Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology. 1996 Nov;137(11):5109-18
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6403291
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Gene symbol:
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Col10a1
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Gene name:
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collagen, type X, alpha 1
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Probe:
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type X collagen cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Tissue fixed in 10% formalin.
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Results
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Specimen
1c:
embryonic day 15.5
(more )
Specimen
1c:
(close )
Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: femur |
Present |
Regionally restricted |
1c
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Expressed in hypertrophic chondrocytes in the center of the bone.
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TS23: tibia |
Present |
Regionally restricted |
1c
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Expressed in hypertrophic chondrocytes in the center of the bone.
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Specimen
1d:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pthlhtm1Hmk/Pthlhtm1Hmk
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: femur |
Present |
Regionally restricted |
1d
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Expressed in hypertrophic chondrocytes in the center of the bone. In the mutant this region is longer than in wild type.
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TS23: tibia |
Present |
Regionally restricted |
1d
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Expressed in hypertrophic chondrocytes in the center of the bone. In the mutant this region is longer than in wild type.
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Specimen
7Ba:
embryonic day 16.5
(more )
Specimen
7Ba:
(close )
Genetic Background:
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involves: C57BL/6
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: hindlimb long bone |
Present |
Regionally restricted |
7Ba
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Expressed in hypertrophic chondrocytes.
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Specimen
7Bd:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pthlhtm1Hmk/Pthlhtm1Hmk
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: hindlimb long bone |
Present |
Regionally restricted |
7Bd
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Expressed in hypertrophic chondrocytes.
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