Myc Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:272568 Munoz-Martin N, et al., Myc is dispensable for cardiomyocyte development but rescues Mycn-deficient hearts through functional replacement and cell competition. Development. 2019 Feb 1;146(3):dev170753
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6403266
Gene symbol:	Myc
Gene name:	myelocytomatosis oncogene

Antibody:	anti-myc
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

2F ©

Specimen 2F: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		2F	Clearly identified a nuclear signal in cardiomyocytes. This contradicted with the RNA in situ and knock-in data.

2G ©

Specimen 2G: embryonic day 10.5; Mycn^tm1Psk/Mycn^tm1Psk, Nkx2-5^tm2(cre)Rph/Nkx2-5⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		2G	Same as the wild-type, indicating the staining was not due to cross-reaction with Mycn and nonspecificity of unknown origin.

S1A left ©

Specimen S1A/B Wt: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		S1A left , S1B lower left , S1B upper left	Clearly identified a nuclear signal in cardiomyocytes. This contradicted with the RNA in situ and knock-in data.

S1A right ©

Specimen S1A/B Mycn-cKO: embryonic day 10.5; Mycn^tm1Psk/Mycn^tm1Psk, Nkx2-5^tm2(cre)Rph/Nkx2-5⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		S1B upper right , S1B lower right , S1A right	Same as the wild-type, indicating the staining was not due to cross-reaction with Mycn and nonspecificity of unknown origin.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24