Probe:	Laf4 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Two probes were used in these studies, one to the coding region and one to the non-coding region. Both produced the same results.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		3A/B	This is a main site of expression.
TS21: cerebral cortex	Present		3A/B	This is a main site of expression.
TS21: thalamus	Present		3A/B	This is a main site of expression.
TS21: hypothalamus	Present		3A/B	Lesser expression is seen in this site.
TS21: midbrain	Present		3A/B	Lesser expression is seen in this site.
TS21: cerebral cortex subventricular zone	Present		3A/B	Most expression in the cortex is confined here.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		3C/D
TS23: cortical plate	Present		3C/D
TS23: amygdala	Present		3C/D
TS23: thalamus	Present	Non-Uniform	3C/D	Stronger expression is seen in the ventral part of the thalamus; weak expression is seen in the remainder.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		3E/F

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		3G/H
TS28: hippocampus	Present		3G/H
TS28: piriform cortex	Present		3G/H

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present
TS28: hippocampus	Present
TS28: piriform cortex	Present

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		4A/B	This is a main site of expression.
TS21: cerebral cortex	Present		4A/B	This is a main site of expression.
TS21: thalamus	Present		4A/B	This is a main site of expression.
TS21: hypothalamus	Present		4A/B	Lesser expression is seen in this site.
TS21: midbrain	Present		4A/B	Lesser expression is seen in this site.
TS21: limb cartilage condensation	Present		4A/B
TS21: rib cartilage condensation	Present		4A/B
TS21: vertebral cartilage condensation	Present		4A/B
TS21: nasal septum	Present		4A/B	Expression is seen in the cartilage.
TS21: cochlea	Present		4A/B
TS21: lung	Present		4A/B
TS21: primitive bladder	Present		4A/B
TS21: renal tubule	Present		4A/B
TS21: heart	Absent		4A/B
TS21: liver	Absent		4A/B
TS21: thymus primordium	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		4D/E	Expression is seen in migrating cells.
TS26: cerebral cortex intermediate zone	Present		4D/E
TS26: basal ganglia	Absent		4D/E
TS26: inferior colliculus	Present		4D/E
TS26: superior colliculus	Absent		4D/E

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		5A/B
TS26: piriform cortex	Present		5A/B

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		5C/D	In the mutant expression is downregulated in the lateroventral part of the cerebral cortex.
TS26: piriform cortex	Absent		5C/D

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Graded	5E	There is a gradient of expression from upper to deep layers.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Graded	5F	In the mutant there is a gradient of expression from deep to upper layers, the inverse of what is seen in wild type.