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Gene Expression Data
Assay Details
Assay
Reference: J:196346 Burgold T, et al., The H3K27 demethylase JMJD3 is required for maintenance of the embryonic respiratory neuronal network, neonatal breathing, and survival. Cell Rep. 2012 Nov 29;2(5):1244-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6403157
Gene symbol: Tacr1
Gene name: tachykinin receptor 1
Antibody: Anti-NK1R (S8305)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 4A Jmjd3+/+: embryonic day 18.5 (more )
Note: Double labeled: magenta - Tacr1; green - Sst (assay MGI:6403159).
Structure Level Pattern Image Note
TS26: brainstem nucleus Present Regionally restricted 4A Jmjd3+/+ NK1R , 4A Jmjd3+/+ Merge Expression was detected in neurons defining the pre-Botzinger complex. Coexpression with Sst was detected in a subset of neurons.
TS26: medulla oblongata Present Regionally restricted 4A Jmjd3+/+ NK1R , 4A Jmjd3+/+ Merge Expression was detected in neurons defining the pre-Botzinger complex. Coexpression with Sst was detected in a subset of neurons.
Specimen 4A Jmjd3-/-: embryonic day 18.5; Kdm6bGt(XB814)Byg/Kdm6bGt(XB814)Byg (more )
Note: Double labeled: magenta - Tacr1; green - Sst (assay MGI:6403159).
Structure Level Pattern Image Note
TS26: brainstem nucleus Weak Diffuse 4A Jmjd3-/- Merge , 4A Jmjd3-/- NK1R Immunopositive neurons were present in the mutant, but they were significantly fewer than in the wild type, with a much weaker density and a diffuse, unstructured distribution of signal. This was particularly prominent for the double labeled neurons.
TS26: medulla oblongata Weak Diffuse 4A Jmjd3-/- Merge , 4A Jmjd3-/- NK1R Immunopositive neurons were present in the mutant, but they were significantly fewer than in the wild type, with a much weaker density and a diffuse, unstructured distribution of signal. This was particularly prominent for the double labeled neurons.
Specimen 4E: embryonic day 18.5 (more )
Note: Double labeled: red - Tacr1; blue - Kdm6b (assay MGI:6403162).
Structure Level Pattern Image Note
TS26: brainstem nucleus Present Regionally restricted 4E Expression was detected in neurons defining the pre-Botzinger complex.
TS26: medulla oblongata Present Regionally restricted 4E Expression was detected in neurons defining the pre-Botzinger complex.
Specimen 4G Jmjd3+/+: embryonic day 16.5 (more )
Note: Double labeled: magenta - Tacr1; green - Sst (assay MGI:6403159).
Structure Level Pattern Image Note
TS24: brainstem nucleus Present Regionally restricted 4G Jmjd3+/+ Merge , 4G Jmjd3+/+ NK1R Expression was detected in the emerging pre-Botzinger complex.
TS24: medulla oblongata Present Regionally restricted 4G Jmjd3+/+ Merge , 4G Jmjd3+/+ NK1R Expression was detected in the emerging pre-Botzinger complex.
Specimen 4G Jmjd3-/-: embryonic day 16.5; Kdm6bGt(XB814)Byg/Kdm6bGt(XB814)Byg (more )
Note: Double labeled: magenta - Tacr1; green - Sst (assay MGI:6403159).
Structure Level Pattern Image Note
TS24: brainstem nucleus Present Regionally restricted 4G Jmjd3-/- NK1R , 4G Jmjd3-/- Merge Expression appeared largely normal in the emerging pre-Botzinger complex at E16.5.
TS24: medulla oblongata Present Regionally restricted 4G Jmjd3-/- NK1R , 4G Jmjd3-/- Merge Expression appeared largely normal in the emerging pre-Botzinger complex at E16.5.
Specimen 5C Jmjd3-/-: embryonic day 18.5; Kdm6bGt(XB814)Byg/Kdm6bGt(XB814)Byg (more )
Note: Double labeled: magenta - Tacr1; green - Sst (assay MGI:6403159).
Structure Level Pattern Image Note
TS26: brainstem nucleus Weak Diffuse 5C Jmjd3-/- NK1R , 5C Jmjd3-/- Merge
TS26: medulla oblongata Weak Diffuse 5C Jmjd3-/- NK1R , 5C Jmjd3-/- Merge
Specimen S3C Jmjd3+/+: embryonic day 18.5 (more )
Note: Double labeled: magenta - Tacr1; green - Phox2b (assay MGI:6403178).
Structure Level Pattern Image Note
TS26: parafascicular nucleus Present S3C Jmjd3+/+ NK1R
TS26: medulla oblongata Present Regionally restricted S3C Jmjd3+/+ NK1R Expression was detected in the retrotrapezoid nucleus.
Specimen S3C Jmjd3-/-: embryonic day 18.5; Kdm6bGt(XB814)Byg/Kdm6bGt(XB814)Byg (more )
Note: Double labeled: magenta - Tacr1; green - Phox2b (assay MGI:6403178).
Structure Level Pattern Image Note
TS26: parafascicular nucleus Present S3C Jmjd3-/- NK1R Expression was normal in the mutant.
TS26: medulla oblongata Present Regionally restricted S3C Jmjd3-/- NK1R Expression was detected in the retrotrapezoid nucleus, as in wild type
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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