Antibody:	PCD-1
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Present	Regionally restricted	1A	Expression associated with surface epithelium of gonadal ridge and with several layers of cells deep to surface epithelium.
TS17: peritoneum	Present	Regionally restricted	1A	Expression in coelomic epithelium covering body wall and abdominal organs.

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Present	Regionally restricted	1B	Expression in subepithelial cells located in area bordered by surface epithelium, attachment of dorsal mesentery and dorsal aorta, and anterior to mesonephros.
TS17: mesonephros	Absent		1B
TS17: nephric duct	Present	Regionally restricted	1B	Expression in epithelilial cells of Wolffian duct.

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Present	Regionally restricted	1C	Expression associated with surface epithelium of gonadal ridge and in subepithelial cells - several layers of cells deep to surface epithelium - located in area bordered by surface epithelium, attachment of dorsal mesentery and dorsal aorta, and anterior to mesonephros.
TS17: mesonephros	Absent		1C
TS17: peritoneum	Present	Regionally restricted	1C	Expression in coelomic epithelium covering body wall and abdominal organs.
TS17: nephric duct	Present	Regionally restricted	1C	Expression in epithelial cells of Wolffian duct.

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present	Regionally restricted	1D	Expression localized to cells of protruding gonadal ridge.

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present	Regionally restricted	1E	Expression localized to cells of protruding gonadal ridge.
TS19: mesonephros	Absent		1E
TS19: peritoneum	Present	Regionally restricted	1E	Expression in coelomic epithelium.
TS19: nephric duct	Present	Regionally restricted	1E	Expression in epithelial cells of Wolffian duct.

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present	Regionally restricted	1F	Expression localized to cells of protruding gonadal ridge.
TS19: mesonephros	Absent		1F
TS19: peritoneum	Present	Regionally restricted	1F	Expression in coelomic epithelium.
TS19: nephric duct	Present	Regionally restricted	1F	Expression in epithelial cells of Wolffian duct.

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Uniform	1G	Expression uniformly distributed throughout expanded cellular mass of indifferent gonad.
TS20: mesonephros	Absent		1G
TS20: nephric duct	Present		1G
TS20: peritoneum	Present		1G

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Uniform	1H	Expression uniformly distributed throughout expanded cellular mass of indifferent gonad.
TS20: mesonephros	Absent		1H
TS20: nephric duct	Present		1H
TS20: peritoneum	Present		1H

Structure	Level	Pattern	Image	Note
TS20: primary sex cord	Present		2A	Expression localized to aggregates of cells forming gonadal cords.
TS20: testis	Present	Regionally restricted	2A	Expression absent from region just deep to surface epithelium - site of future tunica albuginea.
TS20: coelomic epithelium of testis	Present		2A	Expression associated with surface epithelium.
TS20: mesonephric tubule of male	Present	Regionally restricted	2A	Expression associated with epithelial cells of mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Uniform	2B	Expression more uniformly distributed throughout gonad.
TS20: surface epithelium of ovary	Present		2B
TS20: mesonephric tubule of female	Present	Regionally restricted	2B	Expression associated with epithelial cells of mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		2C	Expression in testicular cords becoming progressively organized.

Structure	Level	Pattern	Image	Note
TS21: ovary	Weak		2D	Expression dramatically reduced.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		2E	Expression in testicular cords becoming progressively organized.

Structure	Level	Pattern	Image	Note
TS21: ovary	Weak		2F	Authors report low expression.

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present		2G	Expression in testicular cords becoming progressively organized.
TS22: mesonephric tubule of male	Present		2G	Expression in epithelial cells of mesonephric tubules.
TS22: nephric duct of male	Present	Regionally restricted	2G	Expression in epithelial cells of Wolffian duct.

Structure	Level	Pattern	Image	Note
TS22: surface epithelium of ovary	Present		2H	Only expression associated with ovary were epithelial cells on surface of ovary.
TS22: nephric duct of female	Present	Regionally restricted	2H	Expression in epithelial cells of Wolffian duct.

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Uniform	3A	Uniform expression throughout testis.

Structure	Level	Pattern	Image	Note
TS21: testis	Present	Regionally restricted	3C	Large numbers of cells in developing cords express both P-cadherin (Cdh3) and MIS (Amh).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Sertoli cell	Sertoli cell	Present	Regionally restricted	3E	Expression outlined Sertoli cells.
TS26: germ cell of testis		Absent		3E	No expression in spaces within cords occupied by germ cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: coelomic epithelium of testis		Present		5B	Uniform expression by somatic cells in testis.
TS20: Sertoli cell	Sertoli cell	Present		5B	Uniform expression by somatic cells in testis.

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present		5D	Expression restricted to cords.
TS22: seminiferous cord	Present		5D	Expression restricted to cords.
TS22: interstitium of the testis	Absent		5D	No expression in interstitial spaces.
TS22: coelomic epithelium of testis	Present		5D

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Sertoli cell	Sertoli cell	Present	Regionally restricted	5F	Expression associated with Sertoli cells occupying base of cords.
TS26: testis		Present	Regionally restricted	5F
TS26: germ cell of testis		Absent		5F
TS26: surface epithelium of testis		Present		5F