Antibody:

MEC 13.3

Structure	Level	Pattern	Image	Note
TS15: intersomitic blood vessel	Present		2_E9.5 Ang1-/-

Structure	Level	Pattern	Image	Note
TS15: intersomitic blood vessel	Present		2_E9.5 Ang1+/+

Structure	Level	Pattern	Image	Note
TS17: intersomitic blood vessel	Present		2_E10.5 Ang1-/-

Structure	Level	Pattern	Image	Note
TS17: intersomitic blood vessel	Present		2_E10.5 Ang1+/+

Structure	Level	Pattern	Image	Note
TS19: intersomitic blood vessel	Ambiguous		2_E11.5 Ang1-/-	Authors report intersomitic vessels appear to regress with age in Ang1-/- embryos.

Structure	Level	Pattern	Image	Note
TS19: intersomitic blood vessel	Present		2_E11.5 Ang1+/+

Structure	Level	Pattern	Image	Note
TS20: blood vessel endothelium	Present		2_E12.5 Ang1-/-

Structure	Level	Pattern	Image	Note
TS20: blood vessel endothelium	Present		2_E12.5 Ang1+/+

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber endocardial lining	Present		3a	Authors report immature and much less intricately folded endocardium in mutant embryos as early at E9.5.
TS15: primitive ventricle endocardial lining	Present		3a	Authors report immature and much less intricately folded endocardium in mutant embryos as early at E9.5.

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber endocardial lining	Present		3b
TS15: primitive ventricle endocardial lining	Present		3b

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber endocardial lining	Present	Regionally restricted	3c	Authors report less intense and more speckled staining compared to hearts from littermate controls.
TS17: primitive ventricle endocardial lining	Present	Regionally restricted	3c	Authors report less intense and more speckled staining compared to hearts from littermate controls.

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber endocardial lining	Present		3d
TS17: primitive ventricle endocardial lining	Present		3d

Structure	Level	Pattern	Image	Note
TS19: ventricle endocardial lining	Weak	Regionally restricted	3e	Only speckled ventricular expression.
TS19: atrium endocardial lining	Absent		3e

Structure	Level	Pattern	Image	Note
TS19: atrium endocardial lining	Strong	Uniform	3f	Intense homogeneous expression in both ventricle and atrium.
TS19: ventricle endocardial lining	Strong	Uniform	3f	Intense homogeneous expression in both ventricle and atrium.

Structure	Level	Pattern	Image	Note
TS17: primitive ventricle endocardial lining	Present	Regionally restricted	3g	Expression in endothelial lining at inside surface of ventricle devoid of trabecula.

Structure	Level	Pattern	Image	Note
TS17: heart trabecula	Present	Regionally restricted	3h	Expression in endothelium lining complex myocardial trabeculations filling the ventricle.

Structure	Level	Pattern	Image	Note
TS16: common atrial chamber endocardial lining	Present	Regionally restricted	3i	Authors report almost collapsed atrial endothelial lining.
TS16: primitive ventricle endocardial lining	Present	Regionally restricted	3i	Authors report less complex ventricular endocardium.

Structure	Level	Pattern	Image	Note
TS16: common atrial chamber endocardial lining	Present		3j
TS16: primitive ventricle endocardial lining	Present		3j

Structure	Level	Pattern	Image	Note
TS17: forebrain vascular element	Present	Regionally restricted	4a	Authors report syncytial vessels.

Structure	Level	Pattern	Image	Note
TS17: forebrain vascular element	Present	Regionally restricted	4b	Authors report a fine vascular network.

Structure	Level	Pattern	Image	Note
TS17: forebrain vascular element	Present	Regionally restricted	4c	Authors report dilated vessels, with large spaces within vessels.

Structure	Level	Pattern	Image	Note
TS17: forebrain vascular element	Present	Regionally restricted	4d	Authors report a fine vascular network.

Structure	Level	Pattern	Image	Note
TS19: head blood vessel	Present	Regionally restricted	4e	Vessels remain simpler and smaller in size with much less distinction in size between large and small vessels. Intermediate-size vessels typically not seen. Reduced number of large vessels, smaller in size and with fewer and straighter branches.

Structure	Level	Pattern	Image	Note
TS19: head blood vessel	Present	Regionally restricted	4f	Extensive vascular branchiang and remodeling into large and small vessels. Authors report large vessels, and highly branched meantering intermediate-size vessels.

Structure	Level	Pattern	Image	Note
TS19: eye	Present	Regionally restricted	4g	Authors report decreased vascular complexity in mutant embryos.

Structure	Level	Pattern	Image	Note
TS19: eye	Present		4h

Structure	Level	Pattern	Image	Note
TS19: gut	Present	Regionally restricted	4i	Authors report decreased vascular complexity in mutant embryos.
TS19: umbilical blood vessel	Present	Regionally restricted	4i	Authors report decreased vascular complexity in mutant embryos.

Structure	Level	Pattern	Image	Note
TS19: gut	Present		4j
TS19: umbilical blood vessel	Present		4j

Structure	Level	Pattern	Image	Note
TS20: intersomitic blood vessel	Ambiguous		4k	Authors report intersomittic vessels have regressed.

Structure	Level	Pattern	Image	Note
TS20: intersomitic blood vessel	Present		4l

Structure	Level	Pattern	Image	Note
TS20: yolk sac blood vessel	Present	Regionally restricted	4m	Authors state it appears as if remodeling of initially homogeneous capillary network to does not occur.

Structure	Level	Pattern	Image	Note
TS20: yolk sac blood vessel	Present		4n