Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:242062
Wolfe AD, et al., STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. Dev Biol. 2017 May 01;425(1):44-57
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:6401479
|
|
Gene symbol:
|
Runx1
|
|
Gene name:
|
runt related transcription factor 1
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
8A:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more  )
Note: 8 somite stage. Double labeled: blue - Runx1 reporter; brown - Stella (Dppa3 assay MGI:6401469).
Specimen
8A:
(close  )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
8 somite stage. Double labeled: blue - Runx1 reporter; brown - Stella (Dppa3 assay MGI:6401469).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive hindgut |
Present |
Regionally restricted |
8A
|
Co-expression with Stella (Dppa3) in some cells of ventral hindgut (hindgut lip and ventral midline of hindgut), but not in others.
|
| TS13: vitelline artery |
Present |
|
8A
|
Co-expression with Stella (Dppa3) in omphalomesenteric artery.
|
Specimen
8B:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 12 somite stage. Double labeled: blue - Runx1 reporter; brown - Foxa2 (assay MGI:6401475).
Specimen
8B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12 somite stage. Double labeled: blue - Runx1 reporter; brown - Foxa2 (assay MGI:6401475).
|
Specimen
8C:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 12 somite stage. Double labeled: blue - Runx1 reporter; brown - Foxa2 (assay MGI:6401475).
Specimen
8C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12 somite stage. Double labeled: blue - Runx1 reporter; brown - Foxa2 (assay MGI:6401475).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: hindgut diverticulum |
Present |
Regionally restricted |
8C
|
Co-expression with Foxa2 in some cells in ventral hindgut. No expression in other Foxa2-expressing cells.
|
| TS13: extraembryonic endoderm |
Present |
Regionally restricted |
8C
|
Co-expression with Foxa2 in extrembryonic visceral endoderm.
|
|
Analysis Tools