Gene Expression Data
Assay Details
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Assay
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Reference:
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J:242062
Wolfe AD, et al., STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. Dev Biol. 2017 May 01;425(1):44-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6401475
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Gene symbol:
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Foxa2
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Gene name:
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forkhead box A2
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Antibody:
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anti-HNF-3beta (sc-9187)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
6A:
embryonic day 7.5
(more  )
Note: Early headfold stage.
Specimen
6A:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: posterior visceral endoderm |
Present |
Regionally restricted |
6A
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Expression in allantois-associated posterior visceral endoderm (aPVE) and intraembryonic posterior visceral endoderm (iPVE), persisting through headfold stages.
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Specimen
6B:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
6B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochord |
Present |
Regionally restricted |
6B
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In posterior region anterior to splanchnopleure fusion to form hindgut, expression in notochord, splanchnopleure and yolk sac extraembryonic visceral endoderm (xve).
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| TS12: splanchnopleure |
Present |
Regionally restricted |
6B
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In posterior region anterior to splanchnopleure fusion to form hindgut, expression in notochord, splanchnopleure and yolk sac extraembryonic visceral endoderm (xve).
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| TS12: yolk sac endoderm |
Present |
Regionally restricted |
6B
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In posterior region anterior to splanchnopleure fusion to form hindgut, expression in notochord, splanchnopleure and yolk sac extraembryonic visceral endoderm (xve).
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Specimen
6C:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
6C:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: hindgut diverticulum endoderm |
Present |
Regionally restricted |
6C
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In region posterior to site of hindgut invagination, expression in hindgut endoderm and yolk sac extraembryonic visceral endoderm (xve).
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| TS12: yolk sac endoderm |
Present |
Regionally restricted |
6C
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In region posterior to site of hindgut invagination, expression in hindgut endoderm and yolk sac extraembryonic visceral endoderm (xve).
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Specimen
8B:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 12 somite stage. Double labeled: brown - Foxa2; blue - Runx1 reporter (assay MGI:6401479).
Specimen
8B:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somite stage. Double labeled: brown - Foxa2; blue - Runx1 reporter (assay MGI:6401479).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive hindgut |
Present |
Regionally restricted |
8B
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Co-expression with Runx1 in some cells of ventral hindgut. Some expressing cells lack Runx expression.
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| TS13: yolk sac endoderm |
Present |
Regionally restricted |
8B
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Co-expression with Runx1 in yolk sac visceral endoderm.
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Specimen
8C:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 12 somite stage. Double labeled: brown - Foxa2; blue - Runx1 reporter (assay MGI:6401479).
Specimen
8C:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somite stage. Double labeled: brown - Foxa2; blue - Runx1 reporter (assay MGI:6401479).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: hindgut diverticulum |
Present |
Regionally restricted |
8C
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Co-expression with Runx1 in ventral hindgut. Some expressing cells lack Runx1 expression.
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| TS13: extraembryonic endoderm |
Present |
Regionally restricted |
8C
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Co-expression with Runx1 in extrembryonic visceral endoderm.
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