Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:242062
Wolfe AD, et al., STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. Dev Biol. 2017 May 01;425(1):44-57
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6401468
|
|
Gene symbol:
|
T
|
|
Gene name:
|
brachyury, T-box transcription factor T
|
|
|
Results
|
Specimen
4A/D:
embryonic day 7.0
(more  )
Note: Early bud stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
4A/D:
(close  )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early bud stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: allantois mesoderm |
Present |
Regionally restricted |
4D
,
4A
|
Expression in allantoic bud at extraembryonic primitive streak (XPS)
|
Specimen
4E/H:
embryonic day 7.0
(more )
Note: Early bud stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
4E/H:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early bud stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: allantois mesoderm |
Present |
Regionally restricted |
4E
,
4H
|
Expression in allantoic bud at extraembryonic primitive streak (XPS). Co-expression with Stella (Dppa3) in occasional cells.
|
Specimen
4I/L:
embryonic day 7.5
(more )
Note: Late headfold stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
4I/L:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Late headfold stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
Regionally restricted |
4I
,
4L
|
Expression in Allantoic Core domain (ACD). Ce-expression with Stella (Dppa3) in some cells.
|
Specimen
4M/P:
embryonic day 7.5
(more )
Note: Late headfold stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
4M/P:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Late headfold stage. Triple labeled: red - T; green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: posterior visceral endoderm |
Present |
Regionally restricted |
4M
,
4P
|
No co-expression with Stella (DPPA3) at allantois-associated posterior visceral endoderm (aPVE).
|
Specimen
LHF stage not shown:
embryonic day 7.5
(more )
Note: Late headfold stage.
Specimen
LHF stage not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Late headfold stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
Single cells |
|
Rare Allantoic Core Domain cells co-expressed T, Stella (Dppa3) and Oct-3/4 (Pou5f1), but only at the LHF stage.
|
Specimen
5A/D:
embryonic day 7.5
(more )
Note: Early headfold stage. Triple labeled: red - T; green - Mixl1 (assay MGI:6401474); blue - DAPI.
Specimen
5A/D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early headfold stage. Triple labeled: red - T; green - Mixl1 (assay MGI:6401474); blue - DAPI.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
Regionally restricted |
5A
,
5D
|
In Allantoic Core Domain (ACD) co-expression with Mixl1 in some cells; other cells lack Mixl1 expression.
|
Specimen
8s stage not shown:
embryonic day 8.5
(more )
Note: 8 somite stage.
Specimen
8s stage not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
8 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: allantois mesenchyme |
Present |
Regionally restricted |
|
Cells co-expressing Mixl1 persisted in ACD/presumptive tailbud mesoderm
|
| TS13: hindgut diverticulum |
Present |
Regionally restricted |
|
Cells co-expressing Mixl1 predominated in hindgut's dorsal component during hindgut elongation.
|
| TS13: notochord |
Present |
Regionally restricted |
|
Cells co-expressing Mixl1 limited to posterior edge of notochord.
|
|
Analysis Tools