Arhgef12 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:85854 Becknell B, et al., Characterization of leukemia-associated Rho guanine nucleotide exchange factor (LARG) expression during murine development. Cell Tissue Res. 2003 Dec;314(3):361-6
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6401410
Gene symbol:	Arhgef12
Gene name:	Rho guanine nucleotide exchange factor 12

Antibody:	anti-LARG
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes:	Fixative was 75% acetone/25% ethanol.

Results

Specimen 1a: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: skin	Absent		1a

Specimen 1b: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: epidermis	Strong		1b

Specimen 1c: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong		1c
TS24: hair follicle	Strong		1c

Specimen 1d: postnatal day 1 (more

)

Structure	Level	Image
TS27: epidermis basal layer	Strong	1d
TS27: epidermis	Present	1d
TS27: hair follicle	Present	1d

Specimen 1e: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: epidermis basal layer	Strong		1e
TS28: hair follicle	Present		1e

Specimen 2a: embryonic day 16.0 (more

)
Note: Double labeled: orange - Arhgef12; red - Acta2 (assay MGI:6401353).

Structure	Level	Pattern	Image	Note
TS24: blood vessel smooth muscle	Present		2a , 2c
TS24: bronchus smooth muscle	Present		2a , 2c

Specimen 3a: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: intestine	Absent		3a

Specimen 3b: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: large intestine	Present	Regionally restricted	3b	Expression is in the circular muscle layer of the colon.
TS22: hindgut mucosa	Present	Regionally restricted	3b	Expression is in the colon mucosal epithelium.

Specimen 3c: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: colon muscularis	Present		3c	Expression includes the circular and longitudinal muscle layers.

Specimen 3d: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: ileum	Present	Regionally restricted	3d	Expression is in the muscle layers of the ileum.
TS27: intestine muscularis	Present	Regionally restricted	3d	Expression is in the muscle layers of the ileum.

Specimen 3e: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: ileum	Present		3e
TS28: intestine muscularis	Present		3e

Specimen 4a: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present		4a
TS24: hair follicle	Present		4a

Specimen not shown E8: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Specimen not shown E10: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: embryo	Absent

Specimen not shown E14: embryonic day 14.0 (more

)

Structure	Level	Pattern	Note
TS22: blood vessel	Present
TS22: bronchus	Present
TS22: ileum	Present	Regionally restricted	Expression is in the muscle layers of the ileum.
TS22: intestine smooth muscle	Present	Regionally restricted	Expression is in the muscle layers of the ileum.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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