Ucp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:283695 Quesada-Lopez T, et al., GPR120 controls neonatal brown adipose tissue thermogenic induction. Am J Physiol Endocrinol Metab. 2019 Nov 1;317(5):E742-E750
Assay type:	RT-PCR
MGI Accession ID:	MGI:6400932
Gene symbol:	Ucp1
Gene name:	uncoupling protein 1 (mitochondrial, proton carrier)

Probe:	Mm00494069_m1
Assay notes:	TaqMan gene expression assay. The mRNA level was normalized to that of the reference control, 18S rRNA.

Results

Image: 4A mRNA

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
E19	E19.0 (a)	TS26: brown fat	Present	0.5 µg; total RNA	C57BL/6N		Not Specified	interscapular fat
E19	E19.0 (a)	TS26: brown fat	Present	0.5 µg; total RNA	C57BL/6N-Ffar4^{tm1(KOMP)Vlcg}/MbpMmucd	Ffar4^{tm1(KOMP)Vlcg}/Ffar4^{tm1(KOMP)Vlcg}	Not Specified	interscapular fat
0.25	P0.25	TS27: brown fat	Present	0.5 µg; total RNA	C57BL/6N		Not Specified	interscapular fat
0.25	P0.25	TS27: brown fat	Present (b)	0.5 µg; total RNA	C57BL/6N-Ffar4^{tm1(KOMP)Vlcg}/MbpMmucd	Ffar4^{tm1(KOMP)Vlcg}/Ffar4^{tm1(KOMP)Vlcg}	Not Specified	interscapular fat
0.5	P0.5	TS27: brown fat	Present	0.5 µg; total RNA	C57BL/6N		Not Specified	interscapular fat
0.5	P0.5	TS27: brown fat	Present (b)	0.5 µg; total RNA	C57BL/6N-Ffar4^{tm1(KOMP)Vlcg}/MbpMmucd	Ffar4^{tm1(KOMP)Vlcg}/Ffar4^{tm1(KOMP)Vlcg}	Not Specified	interscapular fat
1	P1	TS27: brown fat	Present	0.5 µg; total RNA	C57BL/6N		Not Specified	interscapular fat
1	P1	TS27: brown fat	Present (c)	0.5 µg; total RNA	C57BL/6N-Ffar4^{tm1(KOMP)Vlcg}/MbpMmucd	Ffar4^{tm1(KOMP)Vlcg}/Ffar4^{tm1(KOMP)Vlcg}	Not Specified	interscapular fat
21	P21	TS28: brown fat	Present	0.5 µg; total RNA	C57BL/6N		Not Specified	interscapular fat
21	P21	TS28: brown fat	Present (c)	0.5 µg; total RNA	C57BL/6N-Ffar4^{tm1(KOMP)Vlcg}/MbpMmucd	Ffar4^{tm1(KOMP)Vlcg}/Ffar4^{tm1(KOMP)Vlcg}	Not Specified	interscapular fat

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels are significantly reduced compared to those seen in wild type.
(c) Expression levels are reduced compared to those seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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