Antibody:	Human Thrombopoietin R/Tpo R APC-conjugated Antibody
Detection system:	Secondary antibody coupled to DyLight 488
Assay notes:	Data was not shown and summarized in Table 1.

Structure	Level	Pattern	Image	Note
TS26: spinal cord gray matter	Present			Cervical region was tested. Relatively high expression.
TS26: spinal cord white matter	Absent			Cervical region was tested.
TS26: spinal cord cervical region	Present	Regionally restricted		Expressed in the grey matter. Relatively high expression.
TS26: medulla oblongata	Present			r2-r8
TS26: cerebral cortex	Present
TS26: hypothalamus	Present
TS26: inferior colliculus	Present
TS26: medulla oblongata roof	Present
TS26: midbrain tegmentum	Present
TS26: pretectum	Present
TS26: pons	Present			r1
TS26: basal ganglia	Absent
TS26: cerebellar cortex	Absent
TS26: cerebellum Purkinje cell layer	Absent
TS26: cerebellum external granule cell layer	Absent
TS26: cerebellum white matter	Absent
TS26: hippocampal formation	Absent
TS26: midbrain-hindbrain junction	Absent
TS26: preoptic area	Present			Relatively high expression.
TS26: prethalamus	Present			Relatively high expression.
TS26: superior colliculus	Present			Relatively high expression.
TS26: thalamus	Present			Relatively high expression.
TS26: cerebral cortex subventricular zone	Weak
TS26: main olfactory bulb	Present	Regionally restricted		Expressed in the inner layers.
TS26: vestibulo-cochlear ganglion	Present	Regionally restricted		Expressed in the granular cells.

Structure	Level	Pattern	Image	Note
TS27: spinal cord cervical region	Present	Regionally restricted		Expressed in the grey matter. Relatively high expression.
TS27: spinal cord gray matter	Present			Cervical region was tested. Relatively high expression.
TS27: spinal cord white matter	Absent			Cervical region was tested.
TS27: medulla oblongata	Present			Relatively high expression. r2-r8. Expressed as clusters of positive cells.
TS27: cerebellum white matter	Present			Relatively high expression. But the lateral recessus of the IVth ventricle and granule cell streams remained negative.
TS27: pons	Present			r1. Relatively high expression. Expressed as clusters of positive cells.
TS27: cerebral cortex subventricular zone	Present			Relatively high expression.
TS27: hypothalamus	Present			Relatively high expression.
TS27: inferior colliculus	Present			Relatively high expression.
TS27: medulla oblongata roof	Present			Relatively high expression.
TS27: midbrain tegmentum	Present			Relatively high expression.
TS27: preoptic area	Present			Relatively high expression.
TS27: pretectum	Present			Relatively high expression.
TS27: prethalamus	Present			Relatively high expression.
TS27: superior colliculus	Present			Relatively high expression.
TS27: thalamus	Present			Relatively high expression.
TS27: vestibulo-cochlear ganglion	Present			Relatively high expression.
TS27: cerebellar cortex	Absent
TS27: cerebellum Purkinje cell layer	Absent
TS27: cerebellum external granule cell layer	Absent
TS27: hippocampal formation	Absent
TS27: midbrain	Present	Regionally restricted		Expressed in the prepontine hindbrain.
TS27: basal ganglia	Present
TS27: cerebral cortex	Present
TS27: main olfactory bulb	Present

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Present			Cervical region was tested. Relatively high expression.
TS28: spinal cord white matter	Absent			Cervical region was tested.
TS28: spinal cord cervical region	Present	Regionally restricted		Expressed in the grey matter. Relatively high expression.
TS28: medulla oblongata	Present			r2-r8
TS28: basal ganglia	Present
TS28: cerebral cortex	Present
TS28: hypothalamus	Present
TS28: medulla oblongata roof	Present
TS28: preoptic area	Present
TS28: pretectum	Present
TS28: prethalamus	Present
TS28: vestibulo-cochlear ganglion	Present
TS28: pons	Present			r1
TS28: cerebellum white matter	Present
TS28: inferior colliculus	Present
TS28: superior colliculus	Present
TS28: cerebellar cortex	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: cerebral cortex subventricular zone	Absent
TS28: main olfactory bulb	Absent
TS28: midbrain tegmentum	Absent
TS28: midbrain	Present	Regionally restricted		Expressed in the prepontine hindbrain.
TS28: thalamus	Present			Relatively high expression.
TS28: hippocampal formation	Weak

Structure	Level	Pattern	Image	Note
TS28: spinal cord cervical region	Weak	Regionally restricted		Expressed in the grey matter.
TS28: spinal cord gray matter	Weak			Cervical region was tested.
TS28: spinal cord white matter	Absent			Cervical region was tested.
TS28: medulla oblongata	Weak			r2-r8
TS28: vestibulo-cochlear ganglion	Absent
TS28: pons	Present			r1
TS28: cerebellum white matter	Present
TS28: cerebral cortex	Present
TS28: hypothalamus	Present
TS28: midbrain tegmentum	Present
TS28: pretectum	Present
TS28: thalamus	Present
TS28: cerebellar cortex	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: cerebral cortex subventricular zone	Absent
TS28: main olfactory bulb	Absent
TS28: medulla oblongata roof	Absent
TS28: preoptic area	Absent
TS28: midbrain	Present	Regionally restricted		Expressed in the prepontine hindbrain.
TS28: basal ganglia	Weak
TS28: inferior colliculus	Weak
TS28: prethalamus	Weak
TS28: superior colliculus	Weak
TS28: hippocampal formation	Present			Relatively high expression.

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Present			Cervical region was tested. Relatively high expression.
TS28: spinal cord white matter	Absent			Cervical region was tested.
TS28: spinal cord cervical region	Present	Regionally restricted		Expressed in the grey matter. Relatively high expression.
TS28: inferior colliculus	Present			Relatively high expression.
TS28: medulla oblongata roof	Present			Relatively high expression.
TS28: midbrain tegmentum	Present			Relatively high expression.
TS28: preoptic area	Present			Relatively high expression.
TS28: superior colliculus	Present			Relatively high expression.
TS28: thalamus	Present			Relatively high expression.
TS28: vestibulo-cochlear ganglion	Present			Relatively high expression.
TS28: medulla oblongata	Present			Relatively high expression. r2-r8.
TS28: pons	Present			r1. Relatively high expression.
TS28: cerebellar cortex	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum white matter	Absent
TS28: cerebral cortex subventricular zone	Absent
TS28: basal ganglia	Present
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebral cortex	Present
TS28: hippocampal formation	Present
TS28: hypothalamus	Present
TS28: pretectum	Present
TS28: prethalamus	Present
TS28: midbrain	Present	Regionally restricted		Expressed in the prepontine hindbrain.
TS28: main olfactory bulb	Present	Regionally restricted		Expressed in the mantle zone.