Antibody:	Human Thrombopoietin R/Tpo R APC-conjugated Antibody
Detection system:	Secondary antibody coupled to DyLight 488

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Present		1A anti human upper left

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Absent		1A anti human upper right

Structure	Level	Pattern	Image	Note
TS27: pons	Present	Regionally restricted	1A anti human middle left	Expressed in ventro-caudal region.

Structure	Level	Pattern	Image	Note
TS27: pons	Absent		1A anti human middle right

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		1A anti human lower left

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Absent		1A anti human lower right

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Present	Regionally restricted	1B lower left	Expressed in ventro-caudal region.

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Absent		1B lower right

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Absent		2A	The green labelling on the blood vessels is unspecific.

Structure	Level	Pattern	Image	Note
TS23: medulla oblongata	Absent		2B	The green labelling on the blood vessels is unspecific.

Structure	Level	Pattern	Image	Note
TS23: pons	Absent		2C	The green labelling on the blood vessels is unspecific.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present		2D

Structure	Level	Pattern	Image	Note
TS26: cochlear nucleus	Present	Regionally restricted	2E	Expressed in dorsal cochlear nucleus.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	2F	Expressed in medial medulla.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	2G	Expressed in dorsal pons.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex subventricular zone	Present		2H
TS27: cerebral hemisphere white matter	Absent		2H

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Regionally restricted	2I	Expressed in the caudal cortex as a densely packed positive cell cluster.

Structure	Level	Pattern	Image	Note
TS27: thalamus	Present	Regionally restricted	2J	Expressed in caudal thalamus as clusters of positive cells.

Structure	Level	Pattern	Image	Note
TS27: dorsal cochlear nucleus	Present		2K	deep regions of the vestibulo-cochlear nuclei

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Absent		3A
TS28: cerebral cortex	Present	Regionally restricted	3A	Expressed in a few cells in the interface between the developing white matter and the inner cortical plate.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present	Regionally restricted	3B	Expressed in rostral thalamus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		3C

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Present	Regionally restricted	3D	Expressed in the ventral medulla.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	3E	Diffuse labelling in the alveus and in some cells scattered underneath pyramidal cells of CA1.

Structure	Level	Pattern	Image	Note
TS28: prethalamus	Present		3F	ventral thalamus

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		3G

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Present	Regionally restricted	3H	Expressed in the ventral medulla.

Structure	Level	Pattern	Image	Note
TS28: preoptic area	Present		3I

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3J
TS28: cerebellum external granule cell layer	Absent		3J
TS28: cerebellum white matter	Absent		3J

Structure	Level	Pattern	Image	Note
TS28: cochlear nucleus	Present	Regionally restricted	3K	Expressed in the dorsal region.

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Present		3L

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Present	Regionally restricted	4A left	Coexpressed with Rbfox3 in majority positive cells in ventral medulla.

Structure	Level	Pattern	Image	Note
TS28: prethalamus	Present		4A middle	Coexpressed with Rbfox3 in majority positive cells in ventral thalamus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		4A right	Positive cells did not co-express Rbfox3.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Absent		4B left
TS27: cortical plate	Present		4B left	Did not coexpressed with Neurod1.

Structure	Level	Pattern	Image	Note
TS27: cerebellum white matter	Present		4B middle	Did not coexpressed with Neurod1.
TS27: cerebellum external granule cell layer	Absent		4B middle

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		4B right	Did not coexpressed with Neurod1.
TS28: cerebellum external granule cell layer	Absent		4B right
TS28: cerebellum granule cell layer	Absent		4B right

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Present	Regionally restricted	4C left	Expressed in the dorsal medulla, but did not coexpressed with Gfap.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present	Regionally restricted	4C middle	Expressed in the medial thalamus. Did not coexpressed with Gfap.

Structure	Level	Pattern	Image	Note
TS28: cochlear nucleus	Present		4C right	Did not coexpressed with Gfap.

Structure	Level	Pattern	Image	Note
TS20: brain	Absent
TS20: spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS23: brain	Absent
TS23: spinal cord	Absent