Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:50964
Tian H, et al., The hypoxia-responsive transcription factor EPAS1 is essential for catecholamine homeostasis and protection against heart failure during embryonic development. Genes Dev. 1998 Nov 1;12(21):3320-4
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:6400238
|
|
Gene symbol:
|
Epas1
|
|
Gene name:
|
endothelial PAS domain protein 1
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
2A:
(close  )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1tm1Rus
|
|
Age:
|
embryonic day 9.0-9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2B:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1tm1Rus
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2C +/-:
embryonic day 10.5; Epas1tm1Rus/Epas1 +
(more  )
Specimen
2C +/-:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2C -/-:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1tm1Rus
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1tm1Rus
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3B:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1tm1Rus
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3C:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1tm1Rus
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3D:
embryonic day 15.5; Epas1tm1Rus/Epas1 +
(more )
Specimen
3D:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4A:
postnatal month 2.5; Epas1tm1Rus/Epas1 +
(more )
Specimen
4A:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1+
|
|
Age:
|
postnatal month 2.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4B:
postnatal month 2.5; Epas1tm1Rus/Epas1 +
(more )
Specimen
4B:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1+
|
|
Age:
|
postnatal month 2.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: carotid body |
Strong |
Regionally restricted |
4B
|
Expression was detected in the endothelial cells and in catecholamine containing type I cells.
|
Specimen
4C:
postnatal month 2.5; Epas1tm1Rus/Epas1 +
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6)
|
|
Mutant Allele(s):
|
Epas1tm1Rus/Epas1+
|
|
Age:
|
postnatal month 2.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: carotid body |
Strong |
Regionally restricted |
4C
|
Expression was detected in the endothelial cells and in catecholamine containing type I cells.
|
|
Analysis Tools