Tg(RNU6-gRNA:Notch1,KRT14-cas9)2Kesa
Transgene Detail
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| Symbol: |
Tg(RNU6-gRNA:Notch1,KRT14-cas9)2Kesa |
| Name: |
transgene insertion 2, Kei Sakamoto |
| MGI ID: |
MGI:6394107 |
| Synonyms: |
N1cKO |
| Transgene: |
Tg(RNU6-gRNA:Notch1,KRT14-cas9)2Kesa Location: unknown
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| Alliance: |
Tg(RNU6-gRNA:Notch1,KRT14-cas9)2Kesa page
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| Transgene Type: |
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Transgenic (Endonuclease, Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: The transgene construct consists of the following elements: the human small nuclear RNA 6 promoter driving expression of an sgRNA targeting exon 2 of the the Notch1 gene; a human keratin 14 promoter driving squamous epithelium-specific expression of the cas9 gene; poly(A) signal sequences. In mice carying this allele, the Notch1 gene is knocked out in tissues where the KRT14 promoter is active. Two F0 founder mice were found. Founder 2 has a 5 bp deletion (AGCCA), causing a reading frameshift and premature translation termination.
(J:233171)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:233171 Mandasari M, et al., A facile one-step strategy for the generation of conditional knockout mice to explore the role of Notch1 in oroesophageal tumorigenesis. Biochem Biophys Res Commun. 2016 Jan 15;469(3):761-7 |
| All: |
1 reference(s) |
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Analysis Tools
