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Gene Expression Data
Assay Details
Assay
Reference: J:279870 Makino Y, et al., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci Rep. 2019 Apr 15;9(1):6063
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6393963
Gene symbol: Bhlhe41
Gene name: basic helix-loop-helix family, member e41
Antibody: anti-Dec2
Results
Specimen S8 anti-DEC2 (upper): postnatal day 5.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S8_DEC2 _ GENA (upper) , S8_Anti-DEC2 (upper) Most Dec2-expressing cells also express germ cell marker GENA/TRA-98.
Specimen S8 anti-DEC2 (lower): postnatal day 5.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S8_DEC2 _ GENA (lower) , S8_ Anti-DEC2 (lower) Most Dec2-expressing cells also express germ cell marker GENA/TRA-98.
Specimen 3a E18.5: embryonic day 18.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS26: germ cell of testis Ambiguous 3a E18.5 Overlay , 3a E18.5 DEC2
Specimen 3a P1.5: postnatal day 1.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3a P1.5 DEC2 , 3a P1.5 Overlay Specific expression in germ cells starting at P1.5.
Specimen 3a P3.5: postnatal day 3.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS27: germ cell of testis Strong 3a P3.5 Overlay , 3a P3.5 DEC2 Prominent expression at P3.5.
Specimen 3a P5.5: postnatal day 5.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS28: germ cell of testis Strong 3a P5.5 DEC2 , 3a P5.5 Overlay
Specimen 3a P7.5: postnatal day 7.5 (more )
Note: Double labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077).
Structure Level Pattern Image Note
TS28: germ cell of testis Strong 3a P7.5 Overlay , 3a P7.5 DEC2
Specimen 3b: postnatal day 7.5 (more )
Note: Triple labeled: purple - Bhlhe41; green - Gcna (TRA-98; assay MGI:7490077); yellow - Gfra1 (assay MGI:6393964).
Structure Level Pattern Image Note
TS28: spermatogonium Strong Regionally restricted 3b DEC2 , 3b Overlay Significantly higher expression in undifferentiated spermatogonia expressing Gfra1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory