Antibody:	9/30/8-A-4
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Weak	Regionally restricted	2g	Expression was weak in the diaphysis of tibia cartilage.

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Present	Regionally restricted	3f	The immunostaining was similar to that of Aggrecan, which was strong in the diaphysis with less staining toward the epiphysis.

Structure	Level	Pattern	Image	Note
TS23: tibia	Strong	Non-Uniform	4f	Expression was strong in the diaphysis, but it was very weak in the metaphysis and epiphysis. The marginal area of the diaphysis and epiphysis had moderate immunostaining.