Antibody:	12/21/1-C-6
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Weak	Regionally restricted	2f	Weak expression was detected in the tibia cartilage diaphysis.

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Strong	Regionally restricted	3e	Strong expression was detected in the diaphysis with less toward the epiphysis.

Structure	Level	Pattern	Image	Note
TS23: tibia	Strong	Regionally restricted	4e	Strong immunostaining was detected in the diaphysis, but there was less staining toward the epiphysis, and none at the epiphyseal end. No immunostaining was detected in the marginal area of the metaphysis and epiphysis.