Gene Expression Data
Assay Details
Assay
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Reference:
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J:86321
Shibata S, et al., In situ hybridization and immunohistochemistry of versican, aggrecan and link protein, and histochemistry of hyaluronan in the developing mouse limb bud cartilage. J Anat. 2003 Oct;203(4):425-32
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6393924
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Gene symbol:
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Vcan
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Gene name:
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versican
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Antibody:
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Anti-Versican
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1c:
embryonic day 13.5
(more )
Specimen
1c:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2e:
embryonic day 13.5
(more )
Specimen
2e:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3d:
embryonic day 14.5
(more )
Specimen
3d:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: tibia cartilage condensation |
Present |
Regionally restricted |
3d
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Immunostaining was detected in the mesenchyme around the cartilage and in the epiphysis, but there was less toward the diaphysis.
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Specimen
4d:
embryonic day 15.5
(more )
Specimen
4d:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: tibia |
Present |
Regionally restricted |
4d
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Expression was detected in the mesenchyme around the tibial cartilage and still in the epiphyseal end of this cartilage, but not in the metaphysis and diaphysis.
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