Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted	2A	Expressed in the ventral region.
TS13: neural tube	Strong		2A
TS13: allantois	Weak	Single cells	2A

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted	2B	Expressed in the ventral region.
TS13: neural tube	Strong		2B
TS13: allantois	Weak	Single cells	2B

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted	2C	Expressed in the ventral region.
TS13: neural tube	Strong		2C
TS13: allantois	Weak	Single cells	2C

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted	2D	Expressed in the ventral region.

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted	2E	Expressed in the ventral region.
TS13: neural tube	Strong		2E

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	2F	Expressed in the ventral region.
TS15: gut	Present		2F	The anterior boundary of expression was approximately at the level of the anlage of the developing lung.
TS15: neural tube	Present		2F	The anterior boundary of expression was approximately at the level of the anlage of the developing lung.
TS15: somite	Present		2F	The anterior boundary of expression was approximately at the level of the anlage of the developing lung.
TS15: unsegmented mesenchyme	Present		2F

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2G
TS15: somite	Present		2G

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	2H	Expressed in the ventral region.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	2I	Expressed in the ventral region.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	2J	Expressed in the ventral region. Extended further along the anterior-posterior axis as compared to E9.5.
TS17: gut	Present		2J	Stronger expression as compared to E9.5, with an anterior boundary at the level of the anlage of the lung.
TS17: neural tube	Present		2J	Stronger expression as compared to E9.5, with an anterior boundary at the level of the anlage of the lung.
TS17: somite	Present		2J	Stronger expression as compared to E9.5, with an anterior boundary at the level of the anlage of the lung.
TS17: unsegmented mesenchyme	Present		2J	Stronger expression as compared to E9.5.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		2K
TS17: somite	Present		2K
TS17: unsegmented mesenchyme	Present		2K

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	2L	Expressed in the ventral region. Extended further along the anterior-posterior axis as compared to E9.5.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	2M	Expressed in the ventral region.

Structure	Level	Pattern	Image	Note
TS19: gut	Present		2N	Strongly decreased as compared to the earlier developmental stages.
TS19: neural tube	Present		2N	Strongly decreased as compared to the earlier developmental stages.
TS19: somite	Present		2N	Strongly decreased as compared to the earlier developmental stages.
TS19: unsegmented mesenchyme	Present		2N	Strongly decreased as compared to the earlier developmental stages.
TS19: brain	Present	Regionally restricted	2N	Expressed in the ventral region. Strongly decreased as compared to the earlier developmental stages.
TS19: urogenital ridge	Present		2N

Structure	Level	Pattern	Image	Note
TS19: somite	Present		2O	Strongly decreased as compared to the earlier developmental stages.
TS19: unsegmented mesenchyme	Present		2O	Strongly decreased as compared to the earlier developmental stages.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		2P	Strongly decreased as compared to the earlier developmental stages.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2Q	Expressed in the ventral region. Strongly decreased as compared to the earlier developmental stages.

Structure	Level	Pattern	Image	Note
TS20: somite	Trace		3A	Expression decreased markedly such that it was barely detectable.
TS20: unsegmented mesenchyme	Trace		3A	Expression decreased markedly such that it was barely detectable.
TS20: spinal cord floor plate	Strong		3A	The anterior boundary appeared more diffuse than at earlier stages.
TS20: gut	Present		3A	The anterior boundary appeared more diffuse than at earlier stages.
TS20: urogenital ridge	Strong		3A

Structure	Level	Pattern	Image	Note
TS20: urogenital ridge	Strong		3C

Structure	Level	Pattern	Image	Note
TS21: intestine	Present		3E
TS21: metanephros	Present		3E
TS21: olfactory epithelium	Present		3E
TS21: spinal cord floor plate	Present		3E
TS21: sympathetic ganglion	Present		3E
TS21: thymus primordium	Present		3E
TS21: tongue papillary placode	Present		3E
TS21: tooth	Present		3E
TS21: urogenital ridge	Present		3E
TS21: oropharynx	Present	Regionally restricted	3E	Expressed in the mucosa.
TS21: brain	Present		3E	Background staining in wildtype embryos appeared only in the choroid plexus.
TS21: limb	Present	Regionally restricted	3E	Expressed in the inner side.

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present		3G
TS21: sympathetic ganglion	Present		3G
TS21: thymus primordium	Present		3G
TS21: urogenital ridge	Present		3G

Structure	Level	Pattern	Image	Note
TS22: brain	Present		3I	Background staining in wildtype littermates was found in some area of the brain.
TS22: eyelid	Present		3I
TS22: hair follicle	Present		3I
TS22: intestine	Present		3I
TS22: limb	Present		3I
TS22: olfactory epithelium	Present		3I
TS22: oropharynx	Present		3I
TS22: ovary	Present		3I
TS22: spinal cord floor plate	Present		3I
TS22: sympathetic ganglion	Present		3I
TS22: thymus primordium	Present		3I
TS22: tongue papillary placode	Present		3I
TS22: tooth	Present		3I
TS22: metanephros	Absent		3I

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		3K
TS22: ovary	Present		3K

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Strong		4A

Structure	Level	Pattern	Image	Note
TS20: early nephron	Strong	Regionally restricted	4B	Strong expression in the tubuli of the metanephros.
TS20: urogenital ridge	Weak	Diffuse	4B	very faint

Structure	Level	Pattern	Image	Note
TS21: cerebral aqueduct	Present	Regionally restricted	5A	Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Strong	Non-Uniform	5B	The outer strong expression was not distributed evenly in all cells.

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Strong	Non-Uniform	5B'	The outer strong expression was not distributed evenly in all cells.

Structure	Level	Pattern	Image	Note
TS21: oropharynx	Present	Regionally restricted	5C	Expressed in the epithelium.
TS21: tongue papillary placode	Present		5C

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Moderate	Regionally restricted	5D	Expressed at the basal level.

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Present	Regionally restricted	5E	More prominent expression in the cortex than in the medulla.

Structure	Level	Pattern	Image	Note
TS21: tooth	Present		5F	Weaker expression compared to the strong staining in the cells of the floor plate.

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Strong		5G

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Present	Regionally restricted	5H	Expressed in an inner cell layer.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	5I	Expressed in the tubuli.

Structure	Level	Pattern	Image	Note
TS22: sympathetic ganglion	Present		6A

Structure	Level	Pattern	Image	Note
TS22: eyelid epithelium	Present		6B	Predominantly located at the basal side.

Structure	Level	Pattern	Image	Note
TS22: oropharynx	Present	Regionally restricted	6C	Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		6D

Structure	Level	Pattern	Image	Note
TS22: tooth	Present	Widespread	6E	More prominent expression and extended throughout the developing teeth primordia.

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Present	Regionally restricted	6F	More prominent expression in the cortex than in the medulla.

Structure	Level	Pattern	Image	Note
TS22: nasal gland	Present	Regionally restricted	6G	Expressed in the tubuli of serous glands associated with the nasal septum.

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present	Single cells	6H	Spotted expression within single cells.

Structure	Level	Pattern	Image	Note
TS22: spinal cord floor plate	Present		6I

Structure	Level	Pattern	Image	Note
TS22: limb mesenchyme	Present	Regionally restricted	6J	Staining on the inner side of the limbs was located under the dermis in mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present	Regionally restricted	6K	Expressed in single cells in the outer epithelium of the intestinal wall.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present	Regionally restricted	6K'	Expressed in single cells in the outer epithelium of the intestinal wall.

Structure	Level	Pattern	Image	Note
TS22: ovary	Present		6L
TS22: metanephros	Absent		6L

Structure	Level	Pattern	Image	Note
TS22: hindgut epithelium	Strong	Widespread	6M

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus	Strong		7A

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Strong		7C

Structure	Level	Pattern	Image	Note
TS28: thymus	Strong	Widespread	8A

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Present		8C

Structure	Level	Pattern	Image	Note
TS15: paraxial mesenchyme	Present		S4_left	Expression faithfully reproduced the endogenous expression pattern, although more sensitive.
TS15: future brain	Present	Regionally restricted	S4_left	Expressed in the ventral region. Expression faithfully reproduced the endogenous expression pattern, although more sensitive.