Mycn RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:171621 Kopecky B, et al., Conditional deletion of N-Myc disrupts neurosensory and non-sensory development of the ear. Dev Dyn. 2011 Jun;240(6):1373-90
Assay type:	RNA in situ
MGI Accession ID:	MGI:6392883
Gene symbol:	Mycn
Gene name:	v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived

Probe:	N-Myc probe9
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

6A ©

Specimen 6A: embryonic day 10.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS17: ear	Present	Ubiquitous	6A

6B ©

Specimen 6B: embryonic day 11.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: ear	Present	Ubiquitous	6B

6C ©

6C' ©

6C'' ©

Specimen 6C: embryonic day 12.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	6C , 6C'	Expression was restricted to all sensory epithelia. Expression in the prosensory domains formed a base-to-apex gradient.
TS20: cochlea epithelium	Present		6C'
TS20: saccule epithelium	Strong		6C''
TS20: utricle epithelium	Strong		6C''

6D ©

6D' ©

Specimen 6D: embryonic day 13.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS21: semicircular canal	Present	Regionally restricted	6D' , 6D	Expression was detected in the semicircular canal cristae.

6E ©

6E' ©

6E'' ©

Specimen 6E: embryonic day 14.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Strong		6E , 6E'' , 6E'

6E'' inset ©

Specimen 6E" inset: embryonic day 14.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS22: ductus reuniens	Strong		6E'' inset
TS22: saccule	Strong		6E'' inset

6H ©

6H' ©

6H' inset ©

Specimen 6H: postnatal day 0 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS27: inner ear epithelium	Strong	Regionally restricted	6H	Expression was detected in sensory epithelium.
TS27: organ of Corti	Strong		6H' , 6H , 6H' inset
TS27: saccule	Present		6H , 6H' inset , 6H'
TS27: utricle	Present		6H , 6H' inset , 6H'

6H inset ©

Specimen 6H inset: postnatal day 0 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS27: crista ampullaris	Present	Regionally restricted	6H inset	Expression was detected in horizontal canal crista.

6K ©

Specimen 6K: embryonic day 12.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		6K

6N WT ©

Specimen 6N WT: embryonic day 14.5 (more

)
Note: Fix: 0.4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		6N WT
TS22: rhombic lip	Present		6N WT
TS22: pons	Present	Regionally restricted	6N WT	Expression was detected in the cochlear nucleus.

6N CKO ©

Specimen 6N CKO: embryonic day 14.5; Mycn^tm1Psk/Mycn^tm1Psk, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Fix: 0.4% paraformaldehyde.

Structure	Level	Image	Note
TS22: inferior colliculus	Absent	6N CKO
TS22: rhombic lip	Absent	6N CKO
TS22: pons	Absent	6N CKO	Expression was not detected in the cochlear nucleus in the conditional mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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