Probe:	Htr1f cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present	Graded	4A	Expression is in a medial high to lateral low gradient.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Regionally restricted	4B	Expression is in the proliferative zone of the caudal ganglionic eminence.
TS22: cortical plate	Present	Graded	4B	Expression is in a medial high to lateral low gradient.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Regionally restricted	4C	Expression is in the proliferative zone of the caudal ganglionic eminence.
TS22: cortical plate	Present	Graded	4C	Expression is in a medial high to lateral low gradient.
TS22: dorsal thalamus	Present	Graded	4C	Expression is in a medial high to lateral low gradient.
TS22: amygdala	Present		4C
TS22: dorsal lateral geniculate nucleus	Absent		4C

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Regionally restricted	4D	Expression is in the proliferative zone of the caudal ganglionic eminence.
TS22: cortical plate	Present	Graded	4D	Expression is in a medial high to lateral low gradient.
TS22: dorsal thalamus	Present	Graded	4D	Expression is in a medial high to lateral low gradient.

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present		4E
TS24: cortical plate	Present		4E

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present		4F
TS24: cortical plate	Present		4F
TS24: ganglionic eminence	Absent		4F

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	4G	Expression is in the CA3 region.
TS24: dorsal thalamus	Present	Graded	4G	Expression is in a medial high to lateral low gradient.
TS24: ganglionic eminence	Absent		4G

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	4H	Expression is in the CA3 region.
TS24: amygdala	Present		4H
TS24: dorsal thalamus	Present	Graded	4H	Expression is in a medial high to lateral low gradient.
TS24: dorsal lateral geniculate nucleus	Absent		4H
TS24: ganglionic eminence	Absent		4H

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Strong	Regionally restricted	4I	Expression is in the rostral part of the cortex.
TS27: cortical layer VI	Strong	Regionally restricted	4I	Expression is in the rostral part of the cortex.

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Strong	Regionally restricted	4J	Expression is in the rostral part of the cortex.
TS27: cortical layer VI	Strong	Regionally restricted	4J	Expression is in the rostral part of the cortex.
TS27: ganglionic eminence	Absent		4J

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		4K
TS27: dorsal lateral geniculate nucleus	Absent		4K
TS27: ganglionic eminence	Absent		4K

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present		4L
TS27: hippocampus	Present		4L
TS27: ganglionic eminence	Absent		4L

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		5B

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		5D

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Strong	Regionally restricted	5F	Expression is in the rostral part of the cortex.
TS27: cortical layer VI	Strong	Regionally restricted	5F	Expression is in the rostral part of the cortex.

Structure	Level	Pattern	Image	Note
TS24: paraventricular thalamic nucleus	Strong		6D
TS24: thalamic nucleus	Strong	Regionally restricted	6D	Expression is in the ventromedial thalamic nucleus.
TS24: medial habenular nucleus	Trace		6D

Structure	Level	Pattern	Image	Note
TS20: brain	Absent

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present
TS22: caudate-putamen	Present
TS22: cerebral cortex subventricular zone	Present
TS22: cerebral cortex ventricular layer	Present
TS22: cortical plate	Present
TS22: ganglionic eminence	Present
TS22: globus pallidus	Present
TS22: hippocampus	Present
TS22: lateral habenular nucleus	Present
TS22: lateral preoptic area	Present
TS22: medial habenular nucleus	Present
TS22: paraventricular thalamic nucleus	Present
TS22: piriform cortex	Present
TS22: reticular thalamic nucleus	Present
TS22: ventral lateral geniculate nucleus	Present
TS22: ventral posterior lateral thalamic nucleus	Present
TS22: ventral posterior medial thalamic nucleus	Present
TS22: zona incerta	Present
TS22: thalamic nucleus	Present			Includes the ventrolateral thalamic nucleus, ventromedial thalamic nucleus, and the posterior complex.
TS22: hypothalamus	Present	Regionally restricted		Expression is in the paraventricular hypothalamic nucleus.
TS22: hypothalamus medial zone	Present	Regionally restricted		Expression is in the medial preoptic area.

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present
TS24: caudate-putamen	Present
TS24: cerebral cortex subventricular zone	Present
TS24: cerebral cortex ventricular layer	Present
TS24: cortical plate	Present
TS24: globus pallidus	Present
TS24: hippocampus	Present
TS24: lateral habenular nucleus	Present
TS24: lateral preoptic area	Present
TS24: medial habenular nucleus	Present
TS24: paraventricular hypothalamic nucleus	Present
TS24: paraventricular thalamic nucleus	Present
TS24: piriform cortex	Present
TS24: reticular thalamic nucleus	Present
TS24: ventral lateral geniculate nucleus	Present
TS24: ventral lateral thalamic nucleus	Present
TS24: zona incerta	Present
TS24: thalamic nucleus	Present			Includes the ventromedial thalamic nucleus and the posterior complex.
TS24: preoptic area	Present	Regionally restricted		Expression is in the medial preoptic area.

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present
TS27: caudate-putamen	Present
TS27: cortical layer V	Present
TS27: cortical layer VI	Present
TS27: cortical plate	Present
TS27: globus pallidus	Present
TS27: hippocampus	Present
TS27: paraventricular thalamic nucleus	Present
TS27: piriform cortex	Present
TS27: posterior thalamic group	Present
TS27: reticular thalamic nucleus	Present
TS27: ventral lateral geniculate nucleus	Present
TS27: ventral lateral thalamic nucleus	Present
TS27: ventral posterior lateral thalamic nucleus	Present
TS27: ventral posterior medial thalamic nucleus	Present
TS27: zona incerta	Present
TS27: thalamic nucleus	Present	Regionally restricted		Expression is in the ventromedial thalamic nucleus.