Antibody:	anti-Ldh3
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		4_GV Oocyte	Mainly appears to localize to the cortex of the cell. A lesser extent expression was seen throughout the cytoplasm.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		4_MII Oocyte	Mainly appears to localize to the cortex of the cell. A lesser extent expression was seen throughout the cytoplasm.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage conceptus	Present		4_PN Zygote	Mainly appears to localize to the cortex of the cell. A lesser extent expression was seen throughout the cytoplasm.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		4_2 Cell	Mostly limited to the cortical region of each blastomere. No staining was seen in the juxtaposed cortical regions of each blastomere.
TS2: second polar body	Present		4_2 Cell	Expressed in the cortex, with the exception of the regions apposing the 2 blastomeres where no staining was seen.

Structure	Level	Pattern	Image	Note
TS3: 4-8 cell stage conceptus	Present		4_4-8 Cell	Expressed in the peripheral cortical regions of external blastomeres, whereas little staining was seen in cortical regions where blastomeres were in apposition.

Structure	Level	Pattern	Image	Note
TS4: morula-stage conceptus	Present		4_Morula	Expressed in the peripheral cortical regions of external blastomeres, whereas little staining was seen in cortical regions where blastomeres were in apposition.

Structure	Level	Pattern	Image	Note
TS4: blastocyst-stage conceptus	Present		4_Blastocyst	Reduced cortical and punctate cytoplasmic staining.