Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:139570
Capellini TD, et al., Pbx1/Pbx2 govern axial skeletal development by controlling Polycomb and Hox in mesoderm and Pax1/Pax9 in sclerotome. Dev Biol. 2008 Sep 15;321(2):500-14
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6391081
|
|
Gene symbol:
|
Meis1
|
|
Gene name:
|
Meis homeobox 1
|
|
Probe:
|
Meis1 probe6
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
S2A:
embryonic day 10.5
(more  )
Specimen
S2A:
(close  )
|
Genetic Background:
|
involves: Black Swiss * C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: notochord |
Weak |
|
S2A
|
Low levels of expression in notochord and surrounding levels compared to expression levels in spinal cord.
|
| TS17: paraxial mesenchyme |
Weak |
|
S2A
|
Low levels of expression in notochord and surrounding levels compared to expression levels in spinal cord.
|
| TS17: future spinal cord |
Present |
Regionally restricted |
S2A
|
Expression in spinal cord with higher levels in ventral horns.
|
Specimen
S2B:
embryonic day 11.5
(more )
Specimen
S2B:
(close )
|
Genetic Background:
|
involves: Black Swiss * C57BL/6
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: notochord |
Weak |
|
S2B
|
Low levels of expression in notochord and surrounding levels compared to expression levels in spinal cord.
|
| TS19: paraxial mesenchyme |
Weak |
|
S2B
|
Low levels of expression in notochord and surrounding levels compared to expression levels in spinal cord.
|
| TS19: future spinal cord |
Present |
Regionally restricted |
S2B
|
Expression in spinal cord with higher levels in ventral horns.
|
Specimen
S2C:
embryonic day 12.5
(more )
Specimen
S2C:
(close )
|
Genetic Background:
|
involves: Black Swiss * C57BL/6
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: notochord |
Weak |
|
S2C
|
Low levels of expression in notochord and surrounding levels compared to expression levels in spinal cord.
|
| TS20: paraxial mesenchyme |
Weak |
|
S2C
|
Low levels of expression in notochord and surrounding levels compared to expression levels in spinal cord.
|
| TS20: spinal cord |
Present |
Regionally restricted |
S2C
|
Expression in spinal cord with higher levels in ventral horns.
|
|
Analysis Tools