Probe:	pMCol2a1-2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe was specific for isoform IIA. Strength was indicated in Table 1.

Structure	Level	Pattern	Image	Note
TS22: vitreous humour	Present		6A/D
TS22: neural retina	Present	Regionally restricted	6A/D	Some labeling was detected in the ganglion cell layer.
TS22: neural retinal epithelium	Absent		6A/D
TS22: pigmented retinal epithelium	Strong		6A/D
TS22: optic choroid	Strong	Regionally restricted	6A/D	Strong expression was detected in Bruch's membrane.
TS22: perioptic mesenchyme	Strong	Regionally restricted	6A/D	Expression was detected in sclera.
TS22: conjunctival sac	Present		6A/D
TS22: lens	Present		6A/D

Structure	Level	Pattern	Image	Note
TS22: cornea	Present

Structure	Level	Pattern	Image	Note
TS24: cornea	Strong		7A/C
TS24: pigmented retinal epithelium	Strong		7A/C
TS24: sclera	Strong		7A/C
TS24: vitreous humour	Strong		7A/C
TS24: retina layer	Strong	Regionally restricted	7A/C	Expression was strong in the gangion cell layer, but was not detected in the neuroepithelial layer.
TS24: optic choroid	Strong	Regionally restricted	7A/C	Expression was strong in Bruch's membrane.
TS24: presumptive iris	Very strong		7A/C
TS24: eye anterior segment	Very strong	Regionally restricted	7A/C	Very strong expression was detected in the ciliary body.
TS24: conjunctival sac	Present		7A/C
TS24: lens	Present		7A/C

Structure	Level	Pattern	Image	Note
TS26: pigmented retinal epithelium	Strong		8A/D
TS26: retina ganglion cell layer	Strong		8A/D
TS26: vitreous humour	Strong		8A/D
TS26: neural retina	Strong	Regionally restricted	8A/D	Expression was strong in the ganglion cell layer, but no labeling was detected in the neuroepithelial layer.
TS26: optic choroid	Strong	Regionally restricted	8A/D	Expression was strong in Bruch's membrane.
TS26: ciliary body	Very strong		8A/D
TS26: iris	Very strong		8A/D
TS26: conjunctival sac	Present		8A/D
TS26: lens	Present		8A/D
TS26: sclera	Present		8A/D
TS26: cornea	Absent		8A/D