Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Ago2tm1.1(DhaA*)Aven
Name:	argonaute RISC catalytic subunit 2; targeted mutation 1.1, Andrea Ventura
MGI ID:	MGI:6390570
Synonyms:	Ago2Halo-LSL
Gene:	Ago2  Location: Chr15:72967693-73056777 bp, - strand  Genetic Position: Chr15, 33.92 cM
Alliance:	Ago2tm1.1(DhaA*)Aven page

Germline Transmission:	Earliest citation of germline transmission: J:283893
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Conditional ready, Epitope tag, Inserted expressed sequence) Mutation:    Insertion   Mutation details: The targeting vector is designed to insert a HaloTag cDNA sequence (see below), a TEV protease recognition sequence and an in-frame loxP-STOP-IRES-FLAG-loxP cassette upstream of the first coding exon (exon 1), and an FRT-flanked PGK-neo cassette in intron 1 of the gene. HaloTag is a 33 kDa haloalkane dehalogenase encoded by the DhaA gene from Rhodococcus rhodochrous that has been mutagenized to form an irreversible covalent bond to synthetic chloroalkane ligands. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:283893)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ago2 Mutation:	54 strains or lines available

Original:	J:283893 Li X, et al., High-resolution in vivo identification of miRNA targets by Halo-Enhanced Ago2 Pulldown. bioRxiv. October;
All:	2 reference(s)