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Gene Expression Data
Assay Details
Assay
Reference: J:92839 Nordqvist K, et al., An mRNA differential display strategy for cloning genes expressed during mouse gonad development. Int J Dev Biol. 1997 Aug;41(4):627-38
Assay type: RT-PCR
MGI Accession ID: MGI:6389589
Gene symbol: Hsd3b1
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Probe: Hsd3b1-pC, Hsd3b1-pD
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: Semi-quantitative RT-PCR normalized to HPRT expression.
Results Image: 5C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
11.5 F E11.5 TS19: gonad
TS19: mesonephros 		Weak 0.5 µg; total RNA Parkes Female 14 tail somites.
11.5 M E11.5 TS19: gonad
TS19: mesonephros 		Weak 0.5 µg; total RNA Parkes Male 14 tail somites.
12.5 F E12.5 TS20: mesonephros
TS20: ovary 		Present 0.5 µg; total RNA Parkes Female
12.5 M E12.5 TS20: mesonephros
TS20: testis 		Present 0.5 µg; total RNA Parkes Male
13.5 F E13.5 TS21: mesonephros
TS21: ovary 		Present 0.5 µg; total RNA Parkes Female
13.5 M E13.5 TS21: mesonephros
TS21: testis 		Strong 0.5 µg; total RNA Parkes Male
17.5 F E17.5 TS25: mesonephros
TS25: ovary 		Present (a) 0.5 µg; total RNA Parkes Female
17.5 M E17.5 TS25: mesonephros
TS25: testis 		Strong (b) 0.5 µg; total RNA Parkes Male
Notes:
(a) Expression in female gonads remained stable during development.
(b) Expression was about 20x higher than seen at E11.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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