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Gene Expression Data
Assay Details
Assay
Reference: J:279056 Donovan LJ, et al., Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife. 2019 Jul 29;8:e48788
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6389233
Gene symbol: Tph2
Gene name: tryptophan hydroxylase 2
Antibody: anti-Tph2 (ABN60)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S1 1B: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Lmx1btm1Zfc/Lmx1btm1Zfc, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: dorsal raphe nucleus Present S1_1 B
Specimen S1 1E: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Lmx1btm1Zfc/Lmx1btm1Zfc, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: dorsal raphe nucleus Present Single cells S1_1 E Merge , S1_1 E
Specimen S2 1 D Control: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: raphe nucleus Present S2_1 D Control
Specimen S2 1 D Lmx1bcKO: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Lmx1btm1Zfc/Lmx1btm1Zfc, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: raphe nucleus Present Single cells S2_1 D Lmx1bcKO Expression is in very few cells.
Specimen 6B Control: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: dorsal raphe nucleus Present 6B Control , 6B Control Merged
TS28: median raphe nucleus Present 6B Control , 6B Control Merged
TS28: raphe nucleus Present 6B Control , 6B Control Merged
TS28: pontine nucleus Present Regionally restricted 6B Control , 6B Control Merged Expression is in B9 nuclei.
Specimen 6B Tph2cKO: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Fev-cre)1Esd/0, Tph2tm1Zfc/Tph2tm1Zfc (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: dorsal raphe nucleus Weak 6B Tph2cKO , 6B Tph2cKO Merged There is a 74% reduction in expression in this mutant.
TS28: median raphe nucleus Weak 6B Tph2cKO , 6B Tph2cKO Merged There is a 74% reduction in expression in this mutant.
TS28: pontine nucleus Weak 6B Tph2cKO , 6B Tph2cKO Merged There is a 74% reduction in expression in this mutant.
Specimen 6B Lmx1bcKO: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Lmx1btm1Zfc/Lmx1btm1Zfc, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: dorsal raphe nucleus Present 6B Lmx1bcKO Merged , 6B Lmx1bcKO There is a 79% reduction in expression in this mutant.
TS28: median raphe nucleus Present 6B Lmx1bcKO Merged , 6B Lmx1bcKO There is a 79% reduction in expression in this mutant.
TS28: pontine nucleus Present 6B Lmx1bcKO Merged , 6B Lmx1bcKO There is a 79% reduction in expression in this mutant.
Specimen 6F Control: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Fev-cre)1Esd/0 (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: medulla oblongata Present 6F Control Merged , 6F Control
Specimen 6F Tph2cKO: postnatal month 3; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Fev-cre)1Esd/0, Tph2tm1Zfc/Tph2tm1Zfc (more )
Note: Conditional mutant. Double labeled: green - Tph2; red - Cre reporter (to reveal serotonin axons).
Structure Level Pattern Image Note
TS28: medulla oblongata Weak 6F Tph2cKO , 6F Tph2cKO Merged
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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