Probe:	Mm-Gli1 (311001)
Probe preparation:	Antisense
Visualized with:	Chromogenic
Assay notes:	RNAscope-Red assay.

Structure	Level	Pattern	Image	Note
TS22: tensor veli palatini	Present		2A inset	Expression was plentiful in the cranial neural crest-derived mesenchymal cells surrounding myogenic cells, and a few MHC-positive cells were also Gli1-positive.

Structure	Level	Pattern	Image	Note
TS22: palatoglossus	Present		2B inset	Expression was abundant in the cranial neural crest-derived mesenchymal cells, and only a few Gli1-positive; MHC-positive cells were visible.

Structure	Level	Pattern	Image	Note
TS22: levator veli palatini	Present		2C inset	Expression was abundant in the cranial neural crest-derived mesenchymal cells surrounding myogenic fibers close to the epithelium; almost no Gli1-positive;MHC-positive cells were detectable.

Structure	Level	Pattern	Image	Note
TS22: soft palate	Present	Regionally restricted	2D inset	In the putative region of the palatopharyngeus, abundant expression was present in the cranial neural crest-derived mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS24: tensor veli palatini	Present		2E inset	Overall fewer Gli1-positive cells were observed in the soft palatal region. The majority of the Gli1-positive cells persisted in the cranial neural crest-derived mesenchyme both surrounding the myogenic fibers and adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS24: palatoglossus	Present		2F inset	Overall fewer Gli1-positive cells were observed in the soft palatal region. The majority of the Gli1-positive cells persisted in the cranial neural crest-derived mesenchyme both surrounding the myogenic fibers and adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS24: levator veli palatini	Present		2G inset	Overall fewer Gli1-positive cells were observed in the soft palatal region. The majority of the Gli1-positive cells persisted in the cranial neural crest-derived mesenchyme both surrounding the myogenic fibers and adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS24: soft palate	Present	Regionally restricted	2H inset	Overall fewer Gli1-positive cells were observed in the soft palatal region. The majority of the Gli1-positive cells persisted in the cranial neural crest-derived mesenchyme both surrounding the myogenic fibers and adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS26: tensor veli palatini	Weak	Single cells	2I inset	At E18.5, overall expression remained low, except a few positive cells were detected in the cranial neural crest-derived mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: palatoglossus	Weak	Scattered	2J inset	Scattered expression was detected in cranial neural crest-derived mesenchyme surrounding the muscle cells.

Structure	Level	Pattern	Image	Note
TS26: levator veli palatini	Trace	Single cells	2K inset	The levator veli palatini was nearly absent of positive cells, with only few positive cells present in cranial neural crest-derived mesenchyme close to the epithelium.

Structure	Level	Pattern	Image	Note
TS26: soft palate	Present	Scattered	2L inset	Scattered expression persisted in cranial neural crest-derived mesenchymal cells close to the epithelium.