Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174050
Rakic S, et al., Cortical interneurons require p35/Cdk5 for their migration and laminar organization. Cereb Cortex. 2009 Aug;19(8):1857-69
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6388762
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Gene symbol:
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Gad1
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Gene name:
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glutamate decarboxylase 1
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
1A:
(close  )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * CBA
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Mutant Allele(s):
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Cdk5r1tm1Lht/Cdk5r1tm1Lht
Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Non-Uniform |
1B
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An increased accumulation of GFP-positive cells was observed in the upper portion of the cortex in the mutant.
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| TS28: cortical layer I |
Present |
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1B
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A significant increase in proportion of GFP+ cells accumulated in layer I of mutant mice compared with controls.
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Specimen
1D:
postnatal day 12; Gad1tm1.1Tama/Gad1 +
(more  )
Note: Double labeled: green - Gad1; red - BrdU (administered E13.5).
Specimen
1D:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gad1; red - BrdU (administered E13.5).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Uniform |
1D
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Early-born interneurons (GFP+/BrdU+) occupy the lower layers.
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Specimen
1E:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * CBA
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Mutant Allele(s):
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Cdk5r1tm1Lht/Cdk5r1tm1Lht
Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gad1; red - BrdU (administered E13.5).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Non-Uniform |
1E
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Early born interneurons (GFP+/BrdU+) were in mostly superficial positions.
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| TS28: cortical layer I |
Present |
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1E
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A significant increase in proportion of GFP+/BrdU+ cells accumulated in layer I of mutant mice compared with controls.
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Specimen
1G:
postnatal day 12; Gad1tm1.1Tama/Gad1 +
(more )
Note: Double labeled: green - Gad1; red - BrdU (administered E15.5).
Specimen
1G:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gad1; red - BrdU (administered E15.5).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Uniform |
1G
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Late-born interneurons (GFP+/BrdU+) were confined to the upper cortical layers.
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Specimen
1H:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * CBA
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Mutant Allele(s):
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Cdk5r1tm1Lht/Cdk5r1tm1Lht
Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gad1; red - BrdU (administered E15.5).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Non-Uniform |
1H
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Late born interneurons (GFP+/BrdU+) were found mostly in the lower aspect of the cortex. A substantial accumulation of late-born cells was also observed within and below Level I in the cortex.
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Analysis Tools