Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278736 Cheong A, et al., A null allele of Dnaaf2 displays embryonic lethality and mimics human ciliary dyskinesia. Hum Mol Genet. 2019 Aug 15;28(16):2775-2784
Assay type:	RNA in situ
MGI Accession ID:	MGI:6388404
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 5G: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		5G , 5G'

Specimen 5H: embryonic day 9.5; Dnaaf2^{tm1.1(KOMP)Vlcg}/Dnaaf2^{tm1.1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		5H , 5H'	Staining pattern was normal in the mutant notochord.

Specimen 5I: embryonic day 8.5; Dnaaf2^{tm1.1(KOMP)Vlcg}/Dnaaf2^{tm1.1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		5I' , 5I	Staining pattern was normal in the mutant notochord.

Specimen 5I": embryonic day 8.5; Dnaaf2^{tm1.1(KOMP)Vlcg}/Dnaaf2^{tm1.1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		5I''	Staining pattern was normal in the mutant notochord.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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