Pitx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278736 Cheong A, et al., A null allele of Dnaaf2 displays embryonic lethality and mimics human ciliary dyskinesia. Hum Mol Genet. 2019 Aug 15;28(16):2775-2784
Assay type:	RNA in situ
MGI Accession ID:	MGI:6388402
Gene symbol:	Pitx2
Gene name:	paired-like homeodomain transcription factor 2

Probe:	Pitx2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 5A: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	5A , 5A'	Expression was confined to the left lateral plate mesoderm.

Specimen 5B: embryonic day 8.5; Dnaaf2^{tm1.1(KOMP)Vlcg}/Dnaaf2^{tm1.1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	5B , 5B'	Pitx2 is expressed inappropriately on the right side in 25% of mutant embryos.

Specimen 5C: embryonic day 9.5; Dnaaf2^{tm1.1(KOMP)Vlcg}/Dnaaf2^{tm1.1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from lateral plate	Present	Regionally restricted	5C' , 5C	Pitx2 is expressed inappropriately on the right side in 25% of mutant embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24