Antibody:	anti-SIX4
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	1A	Expressed in a few cells in the periphery.

Structure	Level	Pattern	Image	Note
TS17: hindbrain mantle layer	Present	Regionally restricted	1B	Expressed in most peripheral regions.
TS17: midbrain mantle layer	Present	Regionally restricted	1B	Expressed in most peripheral regions.
TS17: forebrain	Present	Regionally restricted	1B	Expressed in most peripheral regions of the mantle layer.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	1C	Expressed in the peripheral regIon of the mantle layer.

Structure	Level	Pattern	Image	Note
TS19: hindbrain mantle layer	Strong	Regionally restricted	1D	Expressed in most peripheral regions.
TS19: midbrain mantle layer	Strong	Regionally restricted	1D	Expressed in most peripheral regions.
TS19: forebrain	Strong	Regionally restricted	1D	Expressed in most peripheral regions of the mantle layer.

Structure	Level	Pattern	Image	Note
TS19: spinal ganglion	Present		1E	most prominent

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		2A

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		2B	Most prominent.
TS17: facial ganglion	Present		2B	Most prominent.

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present		2C
TS17: cranial nerve	Present	Regionally restricted	2C	Expressed in olfactory nerve.

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present		2D	semilunar ganglion

Structure	Level	Pattern	Image	Note
TS19: hindbrain mantle layer	Strong	Regionally restricted	2E	Expressed in most peripheral regions.
TS19: superior glossopharyngeal ganglion	Present		2E	Increased expression.
TS19: inferior glossopharyngeal ganglion	Present		2E	Increased expression. Petrosal ganglion.
TS19: inferior vagus ganglion	Present		2E	Increased expression. Nodose ganglion.
TS19: superior vagus ganglion	Present		2E	Increased expression. Jugular ganglion.

Structure	Level	Pattern	Image	Note
TS19: spinal ganglion	Present		2F	most prominent

Structure	Level	Pattern	Image	Note
TS17: glossopharyngeal ganglion	Present			Expressed in superior and petrosal ganglion.
TS17: vagus ganglion	Present			Expressed in jugular and nodose ganglion.
TS17: spinal ganglion	Present

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present			Most prominent.
TS19: facial ganglion	Present			Most prominent.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Weak
TS20: facial ganglion	Absent

Structure	Level	Pattern	Image	Note
TS21: hindbrain mantle layer	Absent
TS21: midbrain mantle layer	Absent
TS21: inferior glossopharyngeal ganglion	Ambiguous			Most staining disappeared.
TS21: inferior vagus ganglion	Ambiguous			Most staining disappeared.
TS21: superior glossopharyngeal ganglion	Ambiguous			Most staining disappeared.
TS21: superior vagus ganglion	Ambiguous			Most staining disappeared.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Weak
TS22: spinal ganglion	Weak
TS22: inferior glossopharyngeal ganglion	Ambiguous			Most staining disappeared.
TS22: inferior vagus ganglion	Ambiguous			Most staining disappeared.
TS22: superior glossopharyngeal ganglion	Ambiguous			Most staining disappeared.
TS22: superior vagus ganglion	Ambiguous			Most staining disappeared.