Gene Expression Data
Assay Details
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Assay
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Reference:
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J:278736
Cheong A, et al., A null allele of Dnaaf2 displays embryonic lethality and mimics human ciliary dyskinesia. Hum Mol Genet. 2019 Aug 15;28(16):2775-2784
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6387030
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Gene symbol:
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Dnaaf2
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Gene name:
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dynein, axonemal assembly factor 2
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fix 0.2% glutaraldehyde and 1% formaldehyde. Note: Authors state that the lacZ expression pattern is strikingly different from the Dnaaf2 mRNA expression pattern observed by multiple distinct Dnaaf2 in situ hybridization probes, which indicated that the wide-spread embryonic expression of Dnaaf2 mRNA is not confined to the node (assay MGI:6386816).
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Results
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Specimen
3D E6.5:
(close  )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: node |
Present |
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3D E6.5
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3D E7.0:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: node |
Present |
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3D E7.0
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3D E7.25:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: node |
Present |
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3D E7.25
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3D E7.5:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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3D E7.5
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3D E8.0:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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3D E8.0
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3D E8.5:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
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3D E8.5
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3D E9.5:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Absent |
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3D E9.5
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Specimen
3E E7.5:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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3E E7.5
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3E E7.75:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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3E E7.75
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Expression was detected only in the node, but not in any other tissues.
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Specimen
3E E8.5:
(close )
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Genetic Background:
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C57BL/6N-Dnaaf2tm1.1(KOMP)Vlcg/J
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Mutant Allele(s):
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Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Glutaraldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
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3E E8.5
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Expression was detected only in the node, but not in any other tissues.
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Analysis Tools
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