Antibody:	anti-N-cadherin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Strong		1A
TS15: primitive ventricle cardiac muscle	Strong		1A
TS15: truncus arteriosus	Strong		1A
TS15: endocardial lining	Absent		1A

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Strong		1B
TS15: primitive ventricle cardiac muscle	Strong		1B
TS15: truncus arteriosus	Strong		1B
TS15: endocardial lining	Absent		1B

Structure	Level	Pattern	Image	Note
TS15: somite	Strong		1C	Expressed in all cells of the somites, with particularly strong expression at the lumenal side of the somitic epithelial vesicles.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2A
TS20: cervical ganglion	Present		2A
TS20: facial nerve	Present		2A
TS20: glossopharyngeal nerve	Present		2A
TS20: trigeminal ganglion	Present		2A
TS20: vagus nerve	Present		2A
TS20: hindbrain	Present		2A
TS20: cerebellum primordium	Present	Regionally restricted	2A	Expressed in the intraventricular region.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present		2B
TS20: neural retina	Strong	Regionally restricted	2B	High level expression in the innermost layer, where the first postmitotic neurons are produced, and in the outermost layer. The inner nuclear layer appeared to be uniformly stained at a low level.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		2C
TS20: hindbrain	Present		2C
TS20: midbrain roof plate	Present		2C
TS20: telencephalon	Present		2C
TS20: cerebellum primordium	Present	Regionally restricted	2C	Expressed in the intraventricular region.
TS20: pons	Present		2C

Structure	Level	Pattern	Image	Note
TS20: posterior commissure	Present		2D

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3A	Expressed in the dorsal spinal cord.
TS20: spinal cord floor plate	Present		3A
TS20: spinal ganglion	Present		3A
TS20: spinal nerve	Present		3A

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		3B
TS20: spinal cord marginal layer	Present		3B

Structure	Level	Pattern	Image	Note
TS20: spinal ganglion	Present		3C
TS20: spinal nerve	Present		3C

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	4A	Expressed in the enteric neuroblasts in the umbilical gut and circular ganglion.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	4B	Expressed in the enteric neuroblasts in the umbilical gut, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Strong	Regionally restricted	4C	Expressed in the myenteric plexus.
TS23: gut mesenchyme	Weak	Regionally restricted	4C	Light staining in the submucosal mesenchyme.
TS23: alimentary system smooth muscle	Absent		4C
TS23: gut mucosa	Absent		4C
TS23: gut serosa	Absent		4C

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Strong	Regionally restricted	4D	A few of the intensely stained cells in the myenteric plexus had prominent nucleoli, while others were stained along what appeared to be axonal cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Strong	Regionally restricted	4E	A few of the intensely stained cells in the myenteric plexus had prominent nucleoli, while others were stained along what appeared to be axonal cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Present	Regionally restricted	5A	Expressed in vertebral body as cell surface staining.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Present	Regionally restricted	5B	Expressed in neural arch as cell surface staining.

Structure	Level	Pattern	Image	Note
TS23: hindlimb bone	Present	Regionally restricted	5C	Expressed in the perichondrium and in the hypertrophic cells of the differentiating cartilage.

Structure	Level	Pattern	Image	Note
TS23: hindlimb bone	Strong	Regionally restricted	5D	Expressed in the perichondrium and in the hypertrophic cells of the differentiating cartilage, but not in the proliferative zone.

Structure	Level	Pattern	Image	Note
TS23: hindlimb bone	Strong	Regionally restricted	5E	Expressed in the perichondrium and on the surface of the hypertrophic cells.

Structure	Level	Pattern	Image	Note
TS23: thoracic body wall	Present	Regionally restricted	5F	Expressed in the mesenchyme surrounding the rib.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Weak		6A

Structure	Cell Type	Level	Pattern	Image	Note
TS22: germ cell of testis		Present		6B
TS22: Sertoli cell	Sertoli cell	Absent		6B

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present	Uniform	7A
TS20: lobar bronchus epithelium	Absent		7A
TS20: dorsal aorta	Weak		7A
TS20: esophagus	Weak		7A

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present	Uniform	7B
TS20: lobar bronchus epithelium	Absent		7B

Structure	Level	Pattern	Image	Note
TS20: brain	Present		8A	Similar as in the wild-type.
TS20: heart	Present		8A	Similar as in the wild-type.
TS20: spinal ganglion	Present		8A	Similar as in the wild-type.
TS20: spinal nerve	Present		8A	Similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS22: lung	Present		8B
TS22: perichondrium	Present		8B

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present
TS20: vertebral cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Present	Regionally restricted		Expressed in the stomach myenteric plexus.
TS23: stomach mesenchyme	Present	Regionally restricted		Expressed in the submucosal mesenchyme.
TS23: limb mesenchyme	Present	Regionally restricted		Expressed in the mesenchyme surrounding the limb.