Vcan RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:277788 Xu F, et al., Expression of Different Isoforms of Versican During the Development of Mouse Mandibular First Molars. J Histochem Cytochem. 2019 Jul;67(7):471-480
Assay type:	RT-PCR
MGI Accession ID:	MGI:6386666
Gene symbol:	Vcan
Gene name:	versican

Probe:	Versican-V1-pA, Versican-V1-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Gapdh was used as the control to normalize the expression levels. The relative expression of the isoform is represented as a fold increase over the Gapdh expression.

Results

Image: 3A V1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
E13.5	E13.5	TS21: molar	Present	1 µg; total RNA	ICR		Not Specified	Bud stage.
E14.5	E14.5	TS22: molar	Present	1 µg; total RNA	ICR		Not Specified	Cap stage.
E16.5	E16.5	TS24: molar	Present	1 µg; total RNA	ICR		Not Specified	Early bell stage.
E18.5	E18.5	TS26: molar	Present	1 µg; total RNA	ICR		Not Specified	Late bell stage.
PN1	P1	TS27: molar	Present	1 µg; total RNA	ICR		Not Specified
PN5	P5	TS28: molar	Present	1 µg; total RNA	ICR		Not Specified
PN7	P7	TS28: molar	Present	1 µg; total RNA	ICR		Not Specified

Notes:
(a) Expression level increased from E13.5 to E14.5, and then decreased from E14.5 to E18.5. After birth, expression increased from E18.5 to P1 and from P1 to P5, but the increases were not significant. Expression increased significantly at P7 to the highest level in the stages examined.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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