Antibody:	anti-Versican (GAG-alpha domain) (AB1032)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: dental placode	Absent		1B
TS19: jaw primordium mesenchyme	Absent		1B

Structure	Level	Pattern	Image	Note
TS21: molar epithelium	Weak	Regionally restricted	1E	The central area of the epithelial tooth bud was devoid of GAG-alpha staining.
TS21: molar mesenchyme	Weak		1E

Structure	Level	Pattern	Image	Note
TS22: molar dental papilla	Strong		1H
TS22: molar enamel organ	Weak	Regionally restricted	1H	Slightly positive staining was noted in the inner and outer enamel epithelium.
TS22: stellate reticulum	Absent		1H

Structure	Level	Pattern	Image	Note
TS24: molar dental papilla	Present		1K
TS24: early bell stage tooth	Present	Regionally restricted	1K	Expression was enhanced in the future cusp formation area.

Structure	Level	Pattern	Image	Note
TS26: molar dental papilla	Present	Non-Uniform	1N	Expression gradually increased in the central dental papilla.
TS26: molar mesenchyme	Present	Regionally restricted	1N	Expression was detected in the preodontoblasts at the future cusp formation area, but disappeared in the mesenchymal cells adjacent to the cervical loop.

Structure	Level	Pattern	Image	Note
TS27: molar epithelium	Strong	Regionally restricted	2C , 2B	Strong staining was detected in the cytoplasm of the preameloblasts. There was no staining in the stellate reticulum.
TS27: molar dental pulp	Present	Regionally restricted	2C , 2B	Expression was detected in the pulp cells, but it was fainter than that in E18.5 and gradually increased to the central pulp.
TS27: molar mesenchyme	Strong	Regionally restricted	2C , 2B	Strong expression was detected in the secretory odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar epithelium	Strong	Regionally restricted	2G , 2H	Intense expression was detected in the ameloblasts.
TS28: molar dental papilla	Weak		2H , 2G
TS28: molar mesenchyme	Strong	Regionally restricted	2H , 2G	Intense expression was detected in the odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar dental papilla	Present		2L , 2M
TS28: molar epithelium	Present	Regionally restricted	2M , 2L	Expression was detected in ameloblasts and pre-enamel.
TS28: molar mesenchyme	Present	Regionally restricted	2L , 2M	Expression was detected in cuspal secretory-odontoblasts, while it was reduced in premature-stage odontoblasts. Signal was absent from cells adjacent to Hertwig's epithelial rooth sheath.

Structure	Level	Pattern	Image	Note
TS28: molar mesenchyme	Strong	Regionally restricted	2Q , 2R' , 2R	Intense expression was detected in the odontoblasts.
TS28: molar epithelium	Strong	Regionally restricted	2R , 2R' , 2Q	Intense expression was detected in ameloblasts.
TS28: molar dental pulp	Strong		2Q , 2R' , 2R
TS28: enamel	Moderate		2R , 2R' , 2Q

Structure	Level	Pattern	Image	Note
TS28: molar mesenchyme	Strong	Regionally restricted	2V , 2W' , 2W	Strong signal was detected in the odontoblast lineage cells at the cornal and apical areas.
TS28: molar dental pulp	Strong	Regionally restricted	2V , 2W' , 2W	Strong expression was detected in the dental pulp cells at the apical areas.
TS28: periodontal ligament	Moderate		2W , 2W' , 2V
TS28: gum epithelium	Moderate	Regionally restricted	2V , 2W' , 2W	Expression was detected in gingival epithelial cells.