Gene Expression Data
Assay Details
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Assay
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Reference:
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J:136041
Gage PJ, et al., The canonical Wnt signaling antagonist DKK2 is an essential effector of PITX2 function during normal eye development. Dev Biol. 2008 May 1;317(1):310-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6385662
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Antibody:
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Pitx2 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
7A:
embryonic day 12.5
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: eye anterior segment |
Strong |
Regionally restricted |
7A
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Expression is high throughout mesenchyme of nascent anterior segment.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Dkk2tm1Dwu/Dkk2tm1Dwu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: eye anterior segment |
Strong |
Regionally restricted |
7B
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Expression in indistinguishable at E12.5 between mutant and wild type eyes.
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Specimen
7C:
embryonic day 16.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cornea |
Weak |
Regionally restricted |
7C
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Expression progressively diminishes in cells of the differentiating cornea.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Dkk2tm1Dwu/Dkk2tm1Dwu
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cornea |
Present |
Regionally restricted |
7D
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Expression not noticeably different in cornea endothelium and mesenchymal cells of emerging iridocorneal angles in cells from either genotype.
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Specimen
7E:
embryonic day 16.5
(more )
Specimen
7E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cornea stroma |
Trace |
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7E
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Expression in corneal stroma is significantly lower than at earlier time poirnts.
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Dkk2tm1Dwu/Dkk2tm1Dwu
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cornea stroma |
Strong |
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7F
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High expression persists within corneal stroma of mutants.
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Analysis Tools