Sin3a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:227506 Jimenez R, et al., Maternal SIN3A regulates reprogramming of gene expression during mouse preimplantation development. Biol Reprod. 2015 Oct;93(4):89
Assay type:	RT-PCR
MGI Accession ID:	MGI:6385573
Gene symbol:	Sin3a
Gene name:	transcriptional regulator, SIN3A (yeast)

Probe:	Sin3a-pE, Sin3a-pF
Probe preparation:	labelled with fluorescein
Assay notes:	This assay is a quantitative RT-PCR. Quantification was normalized to GFP.

Results

Image: 1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
GV	P w 6	TS28: primary oocyte	Present	Not Specified; total RNA	CF-1		Female	Germinal vesicle-intact oocytes
MII	P w 6	TS28: secondary oocyte	Present	Not Specified; total RNA	CF-1		Female	Metaphase II oocytes
1C	E0.5 (b)	TS1: 1-cell stage embryo	Present	Not Specified; total RNA	involves: C57BL/6J * CF-1 * DBA/2J		Not Specified	1-cell embryos
2C	E1.0 (b)	TS2: 2-cell stage embryo	Present	Not Specified; total RNA	involves: C57BL/6J * CF-1 * DBA/2J		Not Specified	2-cell embryos
8C	E2.5 (b)	TS3: 8-cell stage embryo	Weak	Not Specified; total RNA	involves: C57BL/6J * CF-1 * DBA/2J		Not Specified	8-cell embryos
BL	E3.5 (b)	TS4: blastocyst	Present	Not Specified; total RNA	involves: C57BL/6J * CF-1 * DBA/2J		Not Specified	blastocysts

Notes:
(a) Decreased expression during maturation reaching a low point at the 8-cell stage before increasing by the blastocyst stage.
(b) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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